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Total phenolic content (TPC), phenolic profiles, and antioxidant activity of free and bound extracts of Sargassum polycystum, obtained by different extraction solvents and hydrolysis methods, were investigated. Aqueous acetone afforded the highest free TPC and antioxidant ability, followed by aqueous ethanol and aqueous methanol. Twelve free phenolic compounds were identified by ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS), including two hydroxycinnamic acids, seven flavonoids, one stilbene, and two phlorotannins. Three to nine different free phenolic compounds were extracted by these solvents with different compositions, including nine by 70% acetone and eight by 70% methanol, 70% ethanol, and 50% ethanol. The highest total content of free phenolic compounds determined by high-performance liquid chromatography-diode array detection was obtained from 70% ethanol.Alkaline hydrolysis afforded higher bound TPC (274.27 mg GAE/100 g DW) and antioxidant ability than acid hydrolysis. Five bound phenolic compounds were characterized by UHPLC-MS and five were released from alkaline hydrolysis, whereas two were released from acid hydrolysis. Total content of bound phenolic compounds released by alkaline hydrolysis was 14.68-fold higher than that by acid hydrolysis. The free and bound TPC, phenolic profiles, and antioxidantThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Asparagopsis taxiformis is a significant source of phenolics. Owing to the incessant demand of green extraction procedures for phenolics from A. taxiformis, ultrasound-assisted extraction (UAE) using deep eutectic solvents (DESs) was optimized. Among the tested DESs, betaine-levulinic acid afforded the highest total phenolic content (TPC). Moreover, the optimal extraction conditions elucidated from single-factor and response surface methodologies comprised a 52.41°C ultrasonic temperature, 46.48% water content of DES, and 26.99 ml/g liquid-to-solid ratio. The corresponding TPC (56.27 mg GAE/100 g DW) and antioxidant ability fitted the predicted values. UAE afforded superior TPC and antioxidant abilities with DESs than with traditional solvents. Using UHPLC-MS, seven phenolic acids, 18 flavonoids, and two bromophenols were identified and quantified. DES-UAE afforded the highest phenolic compound number (26) and sum of contents. These results disclose the high extraction efficiency of DES-UAE for A. taxiformis phenolics and provide a basis for the higher-value application of this species.