Two peptides on display: The self‐assembly of DNA complexes enables the bivalent presentation of phosphopeptides. Flexibility and distance in the ligand arrangement can be adjusted through the choice of appropriate DNA templates. Spatial screening of the tandem SH2 domain of Syk kinase with these probes (see picture) indicated the accessible arrangements of the two homologous binding pockets and the flexibility of the connecting protein linker.
Peptide-oligonucleotide conjugates have frequently been synthesized to improve cellular delivery of antisense or antigene compounds, to allow the immobilization of peptide and protein conjugates on DNA arrays, or to decorate nucleic acid architectures with peptide functions. In such applications, the site of conjugation is of little importance, and peptides have predominantly been appended to one of the terminal ends of the oligonucleotide by using an oxime-, thioether-, or disulfide-linkage or native chemical ligation. We, herein, demonstrate the first coupling of peptides to sequence internal sites. This attachment mode provides better control of the spatial arrangement of peptides presented by self-assembled nucleic acid scaffolds. Internal modification requires special phosphoramidite building blocks that can be used in automated DNA synthesis. For this purpose, Fmoc/StBu-protected cysteine was attached via an aminopropargyl linker to the C5-position of uridine. The rigid triple bond conferred a high reactivity in native chemical ligation reactions of 5-6mer peptide thioesters with up to 15 nucleotides long oligonucleotides. The desired peptide-oligonucleotide conjugates were obtained in high yields after purification. UV melt experiments revealed that the peptide modification does not hamper nucleic acid hybridization. This finding marked an important step in our research program devoted to studies of multivalent presentation of peptides via modular assembly of nucleic acid complexes.
Synthetic phosphopeptides are frequently used as chemical probes to explore protein-protein interactions involved in cellular signal transduction. Most commonly, the solid-phase synthesis of phosphotyrosine-containing peptides is performed by applying the Fmoc-strategy and N-Fmoc-protected tyrosine derivatives bearing acid-labile phospho protecting groups. We observed a side-reaction, the isomerisation at threonine, which furnishes depsipeptides. It is shown that the rate of N-->O-acyl migration depends on the sequence context. Depsipeptides were formed most rapidly when the phosphotyrosine was located in the +2 position. Furthermore, different phosphotyrosine building blocks were compared and a suitable method that provides phosphopeptides in enhanced purity and yield is suggested.
Sonde mit zwei Peptiden: Die Selbstorganisation von DNA‐Komplexen ermöglicht die Präsentation von zwei Phosphopeptiden. Flexibilität und Abstand zwischen diesen Liganden lassen sich durch Wahl geeigneter DNA‐Template einstellen. Ein räumliches Screening der Tandem‐SH2‐Domäne von Syk‐Kinase mit diesen Sonden (siehe Bild) gab Hinweise auf zugängliche Anordnungen der beiden homologen Bindungstaschen und auf die Flexibilität des domänenverbindenden Proteinlinkers.
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