Poly A containing RNA isolated from embryonic chick calvaria was transferred from 6% formaldehyde 0.75% agarose gels to diazobenzyloxymethyl paper and the paper then hybridized to either nick translated pro alpha 1 collagen cDNA clones, pCg1 or pCg54, or to the nick translated pro alpha 2 collagen cDNA clone, pCg45. From the mobilities of the bands hybridizing most strongly to each, pro alpha 2 collagen mRNA was shown to be slightly larger than pro alpha 1 mRNA; they are 5100 and 4900 nucleotides long respectively. pCg54 also hybridized weakly to two bands of lower mobility, corresponding to RNAs 6.4 and 5.6 kb long. Neither pCg54 nor pCg45 hybridized to type II procollagen mRNA in poly A containing RNA isolated from embryonic chick sterna.
in the melt for some time the surface appears to have been "polished" more brightly and uniformly than would be possible by purely mechanical means. The more the metal has been polished before immersion the shorter is the induction period. Thus it appears likely that the induction period represents the removal of surface impurities. The final electrode potential is not affected by the previous treatment of the metal. On the other hand the potential of nickel in aqueous sodium hydroxide depends on the careful reduction and outgassing of the metal before immersion in the solution. Probably the solution of surface impurities occurs rapidly only at temperatures higher than the melting point of sodium hydroxide.
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