Soil biodiversity plays a key role in regulating the processes that underpin the delivery of ecosystem goods and services in terrestrial ecosystems. Agricultural intensification is known to change the diversity of individual groups of soil biota, but less is known about how intensification affects biodiversity of the soil food web as a whole, and whether or not these effects may be generalized across regions. We examined biodiversity in soil food webs from grasslands, extensive, and intensive rotations in four agricultural regions across Europe: in Sweden, the UK, the Czech Republic and Greece. Effects of land-use intensity were quantified based on structure and diversity among functional groups in the soil food web, as well as on community-weighted mean body mass of soil fauna. We also elucidate land-use intensity effects on diversity of taxonomic units within taxonomic groups of soil fauna. We found that between regions soil food web diversity measures were variable, but that increasing land-use intensity caused highly consistent responses. In particular, land-use intensification reduced the complexity in the soil food webs, as well as the community-weighted mean body mass of soil fauna. In all regions across Europe, species richness of earthworms, Collembolans, and oribatid mites was negatively affected by increased land-use intensity. The taxonomic distinctness, which is a measure of taxonomic relatedness of species in a community that is independent of species richness, was also reduced by land-use intensification. We conclude that intensive agriculture reduces soil biodiversity, making soil food webs less diverse and composed of smaller bodied organisms. Land-use intensification results in fewer functional groups of soil biota with fewer and taxonomically more closely related species. We discuss how these changes in soil biodiversity due to land-use intensification may threaten the functioning of soil in agricultural production systems.

Soil organisms have an important role in aboveground community dynamics and ecosystem functioning in terrestrial ecosystems. However, most studies have considered soil biota as a black box or focussed on specific groups, whereas little is known about entire soil networks. Here we show that during the course of nature restoration on abandoned arable land a compositional shift in soil biota, preceded by tightening of the belowground networks, corresponds with enhanced efficiency of carbon uptake. In mid- and long-term abandoned field soil, carbon uptake by fungi increases without an increase in fungal biomass or shift in bacterial-to-fungal ratio. The implication of our findings is that during nature restoration the efficiency of nutrient cycling and carbon uptake can increase by a shift in fungal composition and/or fungal activity. Therefore, we propose that relationships between soil food web structure and carbon cycling in soils need to be reconsidered.

Intensive land use reduces the diversity and abundance of many soil biota, with consequences for the processes that they govern and the ecosystem services that these processes underpin. Relationships between soil biota and ecosystem processes have mostly been found in laboratory experiments and rarely are found in the field. Here, we quantified, across four countries of contrasting climatic and soil conditions in Europe, how differences in soil food web composition resulting from land use systems (intensive wheat rotation, extensive rotation, and permanent grassland) influence the functioning of soils and the ecosystem services that they deliver. Intensive wheat rotation consistently reduced the biomass of all components of the soil food web across all countries. Soil food web properties strongly and consistently predicted processes of C and N cycling across land use systems and geographic locations, and they were a better predictor of these processes than land use. Processes of carbon loss increased with soil food web properties that correlated with soil C content, such as earthworm biomass and fungal/bacterial energy channel ratio, and were greatest in permanent grassland. In contrast, processes of N cycling were explained by soil food web properties independent of land use, such as arbuscular mycorrhizal fungi and bacterial channel biomass. Our quantification of the contribution of soil organisms to processes of C and N cycling across land use systems and geographic locations shows that soil biota need to be included in C and N cycling models and highlights the need to map and conserve soil biodiversity across the world.

Summary1. Ecological stoichiometry predicts important control of the relative abundance of the key elements carbon (C), nitrogen (N) and phosphorus (P) on trophic interactions. In a nutrient-poor Amazonian lowland rain forest of French Guiana, we tested the hypothesis that decomposers exploit stoichiometrically diverse plant litter more efficiently, resulting in faster litter decomposition compared to litter with a uniform stoichiometry. 2. In a field experiment in the presence or absence of soil macrofauna, we measured litter mass loss, and N and P dynamics from all possible combinations of leaf litter from four common tree species which were distinctly separated along a C:N and along a N:P gradient. 3. Mean litter mass remaining after 204 days of field exposure varied between 25.2% and 71.3% among litter treatments. Fauna increased litter mass loss by 18%, N loss by 21% and P loss by 14%. Litter species richness had no effect on litter mass loss or nutrient dynamics. In contrast, litter mass and nutrient losses increased with increasing stoichiometric dissimilarity of litter mixtures in presence of fauna, suggesting faster decomposition of a stoichiometrically more heterogeneous litter. 4. However, the effect of stoichiometric dissimilarity was smaller than the strong C quality related litter composition effect and disappeared in the absence of fauna. Increasing proportions of litter that is relatively rich in accessible C compounds (non-structural carbohydrates, phenolics) and relatively poor in recalcitrant C (condensed tannins, lignin), correlated best with litter mass loss irrespective of fauna presence. No correlation was found for any of the nutrient related litter quality parameters and decomposition. 5. Synthesis. Our results suggest that Amazonian decomposer communities studied here are primarily limited by energy, and only secondarily by litter stoichiometry. Tropical tree species might thus influence decomposers and detritivores by the production of litter of specific C quality with potentially important feedback effects on ecosystem nutrient dynamics and availability.

Background: The loss of carbon (C) from agricultural soils has been, in part, attributed to tillage, a common practice providing a number of benefits to farmers. The promotion of less intensive tillage practices and no tillage (NT) (the absence of mechanical soil disturbance) aims to mitigate negative impacts on soil quality and to preserve soil organic carbon (SOC). Several reviews and meta-analyses have shown both beneficial and null effects on SOC due to no tillage relative to conventional tillage, hence there is a need for a comprehensive systematic review to answer the question: what is the impact of reduced tillage intensity on SOC? Methods:We systematically reviewed relevant research in boreo-temperate regions using, as a basis, evidence identified within a recently completed systematic map on the impacts of farming on SOC. We performed an update of the original searches to include studies published since the map search. We screened all evidence for relevance according to predetermined inclusion criteria. Studies were appraised and subject to data extraction. Meta-analyses were performed to investigate the impact of reducing tillage [from high (HT) to intermediate intensity (IT), HT to NT, and from IT to NT] for SOC concentration and SOC stock in the upper soil and at lower depths.Results: A total of 351 studies were included in the systematic review: 18% from an update of research published in the 2 years since the systematic map. SOC concentration was significantly higher in NT relative to both IT [1.18 g/ kg ± 0.34 (SE)] and HT [2.09 g/kg ± 0.34 (SE)] in the upper soil layer (0-15 cm). IT was also found to be significant higher [1.30 g/kg ± 0.22 (SE)] in SOC concentration than HT for the upper soil layer (0-15 cm). At lower depths, only IT SOC compared with HT at 15-30 cm showed a significant difference; being 0.89 g/kg [± 0.20 (SE)] lower in intermediate intensity tillage. For stock data NT had significantly higher SOC stocks down to 30 cm than either HT [4.61 Mg/ ha ± 1.95 (SE)] or IT [3.85 Mg/ha ± 1.64 (SE)]. No other comparisons were significant. Conclusions:The transition of tilled croplands to NT and conservation tillage has been credited with substantial potential to mitigate climate change via C storage. Based on our results, C stock increase under NT compared to HT was in the upper soil (0-30 cm) around 4.6 Mg/ha (0.78-8.43 Mg/ha, 95% CI) over ≥ 10 years, while no effect was detected in the full soil profile. The results support those from several previous studies and reviews that NT and IT increase SOC in the topsoil. Higher SOC stocks or concentrations in the upper soil not only promote a more productive soil with higher biological activity but also provide resilience to extreme weather conditions. The effect of tillage practices on total SOC stocks will be further evaluated in a forthcoming project accounting for soil bulk densities and crop yields. Our findings can hopefully be used to guide policies for sustainable management of agricultural soils.