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IGC does not simply measure the high-energy sites in the packed column, but equally there is a complex process that results in measured data on mixtures not being a weighted mean of the surface energy of the two components.

Milling had made the surface energy similar to that of the amorphous material in a manner that was very different to a physical mixture of the same amorphous content. The milled material will have similar interfacial interactions to the 100% amorphous material.

The reÑection absorption infrared (RAIR) spectrum of the adsorbed ethyl moiety produced by the (C 2 H 5 ) dissociative adsorption of ethane from a supersonic molecular beam onto Pt(111) at 150 K is reported. The thermal chemistry of the fragment so produced was then followed using a combination of RAIR C 2 H 5 spectroscopy and temperature programmed desorption (TPD). The RAIR spectra indicate the presence of ethylidene and ethylidyne at 250 and 350 K respectively. This implies the stepwise loss of (CHCH 3 ) (CCH 3 ) one and two hydrogen atoms from the ethyl moiety during the heating process. Temperature programmed desorption (TPD) measurements show that hydrogen desorption is not accompanied by desorption of either saturated or unsaturated hydrocarbons or methane into the gas phase. C 2

Implantation of left ventricular assist devices (LVAD)is associated with HLA antibody sensitization. The objective of this study was to determine the specificity of antibodies produced by LVAD recipients using a combination of ELISA, Luminex and microcytotoxicity assays. Fifty-one LVAD patients were studied, from 44 to 838 days post-implantation. No patient developed HLA antibodies, although 24 produced IgG antibodies detectable in both ELISA and Luminex assays. These antibodies manifest as positive reactions with class I and class II wells of the ELISA and also blank wells. In Luminex assays, they produce high MFI readings with the negative control beads. Antibodies were detected 18 to 228 days after implantation. This reactivity was found to be directed against bovine serum albumin (BSA), commonly used to block non-specific binding in ELISA and Luminex assays; absorption of sera with BSA-coated beads completely abrogated reactivity in all solid phase assays, but did not eliminate anti-HLA antibodies in control sera. Ten of the 24 patients have proceeded to transplantation, with a 1-year graft survival of 69%. In conclusion, it appears that implantation of LVADS disrupts immunoregulatory pathways leading to production of anti-albumin antibodies. These can be misinterpreted as anti-HLA antibodies in solid phase assays.