Twenty seven strains of lactic acid bacteria (LAB) were isolated from bakasang, Indonesian traditionalfermented fsh product. In general, LAB have inhibitory activity againts pathogenic bacteria and spoilagebacteria. Screening for antimicrobia activity of isolates were performed with well-diffusion method. Oneisolate that was designed as Pediococcus BksC24 was the strongest against bacteria pathogenic and spoilagebacteria. This strain was further identifed by 16S rRNA gen sequence comparison. Isolates LAB producingantimicrobial agents from bakasang were identifed as Pediococcus acidilactici.
The application of tuba root bioactive extract (Derris elliptica L.) as a natural insecticide on the Crocidolomia binotalis caterpillar on mustard plants (Brassica juncea L.) was carried out. The test results showed that there were significant differences in antifeedant activity of the Crocidolomia binotalis caterpillar on mustard (Brassica juncea L.) plants at various concentrations. The research was carried out in several stages, starting from the tubal root extraction stage, phytochemical testing, preparation of caterpillar tests, testing of antifeedant activity. As the treatment is the level of methanol concentration of 50 ppm, 100 ppm, 500 ppm and 1000 ppm. The parameter observed was the percentage of Feeding Reduction (FR). The test results showed that the best antifeedant activity of Crocidolomia binotalis was at concentrations of 500 ppm and 1000 ppm because it was able to inhibit feeding power or Feeding Reduction of test caterpillars by 15.35% and 32.33%, was able to inhibit the feeding activity of Crocidolomia binotalis.
Langsat fruit (Lansiumdomesticum) sample collected from 4 district in Minahasa, North Celebes. Lactic Acid Bacteria (LAB) isolates were found at that samples. The study aim to examinedthe antibacterial activity of Lactic acid bacteria (LAB) from Langsat fruit sample against pathogenic bacteria Escherichia coli (Gram Negatrive Bacteria) and spoilage bacteria Staphylococcus aureus (Gram Positive Bacteria) using agar well diffusion method.The isolation result obtained 42 isolates of LAB namely 10 isolate were obtained from Southeast Minahasa district, 8 isolates were obtained from the southern district of Minahasa, 12 isolates were obtained from from the Minahasa district, and 12 isolates were obtained from North Minahasa district. Fourty two isolates LAB identified as Lactobacillus genera based on profile matching method were effective against bacteria indicator (pathogenic and spoilage bacteria). The antibacterial activity showed through the formation of a clear zone around well that containing LAB isolates. The diameter inhibition of LAB isolates ranged from 3,0 -17,0 mm. Overall, the isolated LAB showed the remarkable inhibitory effect against both Gram positive (spoilage bacteria) and Gram negative (pathogenic bacteria). However, the spectrum of inhibition was different for the isolates tested. These results suggest that this potent isolates could be used as a natural biopreservativesagent.
Diversity of Lactic acid bacteria (LAB) that have ability to improved number of carnocine on bakasang were determind by application of molecular approach. Molecular characterization of LAB was based on 16S rRNA gene sequencing. The result showed that one isolate lactic acid bacteria (Pediococcus sp.B.5.1) among 9 isolates LAB exhibited the highest ability to improved number of carnocine. The molecular characterization based on 16S rRNA gene sequence showed that the Pediococcus B.5.1 isolate clearly belonged to members of species Pediococcus acidilactici. Therefore, lactic acid bacteria with the high ability to improved number of carnosine could be found from fermented fish bakasang and make bakasang as a functional food.
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