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A method for isolating the mitotic apparatus from dividing sea urchin eggs without the use of ethyl alcohol or of detergents is described. In the present method, the eggs are dispersed directly in a medium containing 1 M (to 1.15 M) sucrose, 0.15 M dithiodiglycol, and 0.001 M Versene at pH 6, releasing the visibly intact mitotic apparatus. The method is designed for studies of enzyme activities, lipid components, and the variables affecting the stability of the apparatus.

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A description of the application of the classical Millon reaction as used to produce a differential stain for gray and white matter of the central nervous system is presented. The use of this technique for a gross anatomic study of the brain of the dog is discussed.