Herein, we declare on the biosynthesis of synthesised silver nanoparticles utilizing the aqueous extract of Origanum Marjorana by reducing 1 mM silver nitrate at room temperature. AgNPs were characterized by using some common techniques such as an ultra violet spectrophotometer, Fourier transforms infrared spectroscopy, X-ray diffraction, and transmission electron microscopy. Morphologically, AgNPs demonstrated a spherical shape with an average particle size distribution of 63-85 nm. FT-IR studies showed the presence of bioactive useful bunches such as phenolic compounds, amines, and aromatic rings are found to be the capping, stabilizing specialists and required for the reduction of silver ions of AgNPs. The measurement of toxicity of AgNPs and the plant extract of O. marjorana against Earias insulana was also discussed. After the treatment of synthesized nanoparticles for two successive seasons, general reduction percentages on the infestation of the green boll caused by E. insulana larvae in the second season was more by 60.4% than the first season which was 44.73%. Synthesized AgNPs treatment was considered more effective than plant extract for controlling E. insulana. General reduction percentages in the infestation of the green boll caused by E. insulana larvae in the first season associated with the treatments with AgNPs and plant extract were 44.73 and 37.53% while the second season record 60.4 and 51.82%; respectively.

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