The (1)H NMR spectra of metabolites in the rat blood are differentially changed after chronic stress. Specific, characteristic metabolic products can be identified by analyses of metabonomics, which lead to interpretation of biological feature of Chinese medicine syndromes. Therefore, metabonomic analysis is an approach with good development prospects to studies of TCM syndromes.
Some common and characteristic metabolites on the anti-CIS of XYP and its modified prescription were obtained. The metabolomics technology is a valuable tool and may be used to identify the specific metabolites and potential biomarkers of therapeutic effect of Chinese medicinal prescriptions.
To determine changes in the plasma metabolic phenotype in rats with chronic immobilization stress (CIS), and attempt to interpretation of metabonomic changes in the rats induced by CIS via determination of characteristic metabolic products and discovery of biomarkers in the rats. Blood was collected from the cardioventricle under anesthesia and detected by using the Fourier variable superconducting nuclear magnetic resonance (NMR) spectromete.The results showed: the PCA analysis of rat plasma 1 H NMR spectra revealed different metabolic spectra between stress and control groups, which was consistent with alterations of in vivo metabolisms in rats under stress stimuli. It can be concluded that: the metabolic phenotype of CIS includes increases in plasma levels of lactic acid, choline, NAC, saturated fatty acid, and decreases in the levels of unsaturated fatty acid, blood sugar, HDL, and the unknown compounds (3.44 ppm). These metabolic components also may be the biomarkers of metabolites induced by CIS.
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