Metabonomic study of syndrome of liver qi stagnation and spleen deficiency in rats

Luo, He-gu; Ding, Jie; Yue, Guang-Xin; Chen, Jiaxu

doi:10.3736/jcim20070315

Cited by 19 publications

(11 citation statements)

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“…TCM theory is intrinsically correlated with metabonomics, so using metabonomics facilitates TCM modernization, such as the essence of "patterns", "pattern identification", and "Bianzhengshizhi (identify patterns and administer treatment)". Preliminary NMR-based metabonomic studies of "patterns" in TCM [11,12] aimed directly at selected "patterns" with few studies published concerning the application of metabonomics to "pattern identification".…”

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confidence: 99%

Metabonomic phenotype and identification of “heart blood stasis obstruction pattern” and “qi and yin deficiency pattern” of myocardial ischemia rat models

Yan

Hao

et al. 2009

SCI CHINA SER C

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The traditional Chinese medicine concepts of "Xinxueyuzuzheng (heart blood stasis obstruction pattern)" and "Qiyinliangxuzheng (qi and yin deficiency pattern)" for myocardial ischemia rat models were constructed in the present study. Endogenous metabolites in rat plasma were analyzed using the GC/TOF-MS-based metabonomic method. Significant metabolic differences were observed between the control and two model groups, and the three groups were distinguished clearly by pattern recognition. Compared with those of the control, the levels of hydroxyproline, threonic acid, glutamine and citric acid were strikingly up- or down-regulated in model rats. The metabolites contributing most to the classification between the two "pattern" rats were identified, such as valine, serine, threonine, ornithine, hydroxyproline, lysine, 2-hydroxybutanoic acid, 3-hydroxybutanoic acid, galactofuranose and inositol. These compounds were indicated as the potential biomarkers. The results suggested that the two "patterns" are involved in dysfunction in oxidative stress, energy metabolism and amino acid metabolism. These findings also provided the substantial foundation for exploring the scientific connotation of these two "Zhengxing (pattern types)" of myocardial ischemia, and "Bianzheng (pattern identification)".

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confidence: 99%

Metabonomic phenotype and identification of “heart blood stasis obstruction pattern” and “qi and yin deficiency pattern” of myocardial ischemia rat models

Yan

Hao

et al. 2009

SCI CHINA SER C

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“…Metabonomics realistically reflects the overall environment of various characteristic changes in humoral metabolites and reveals the overall human metabolic network and the laws governing metabolic changes under the influence of medicine [4]. This property is consistent with the holistic philosophy of TCM and might provide an opportunity to express scientifically the meaning of evidence-based Chinese medicine, such as Chinese medicine syndromes (CMS), preventive treatment, the action of Chinese medicine, Chinese medical formulas (CMF) and acupuncture efficacy [1,5]. In a clinical study, the metabonomics method was applied to study clinical urine samples in patients with suboptimal health with different syndromes [6].…”

Section: Introductionmentioning

confidence: 79%

“…In a clinical study, the metabonomics method was applied to study clinical urine samples in patients with suboptimal health with different syndromes [6]. In nonclinical studies, the metabonomics method has been applied in discussing the syndrome of the stagnation of liver Qi and spleen deficiency and has assisted in revealing the biological changes in the rats with chronic restraint stress-induced syndrome of stagnation of the liver Qi and spleen deficiency [5,6]. The analysis of plasma metabonomics was applied in mini-swines with qi deficiency and blood stasis syndrome from chronic myocardial ischemia [7] and in research on the evaluation of the fever syndrome model in rats [8].…”

Section: Introductionmentioning

confidence: 99%

Plasma metabonomics study on Chinese medicine syndrome evolution of heart failure rats caused by LAD ligation

Qiu

Wang

et al. 2014

BMC Complement Altern Med

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BackgroundChinese medicine syndromes (Zheng) in many disease models are not clearly characterized or validated, and the concepts of Chinese medicine syndromes are confounding and controversial. Metabonomics has been applied to the evaluation and classification of the Chinese medicine syndromes both in clinical and nonclinical studies. In this study, we aim to investigate the evolution of the Chinese medicine syndrome in myocardial infarction induced heart failure and to confirm the feasibility of the Zheng classification by plasma metabonomics in a syndrome and disease combination animal model.MethodsThe heart failure (HF) model was induced by ligation of the left anterior descending coronary artery (LAD) in Sprague–Dawley rats. The rats were divided into the following two groups: the HF model group (LAD ligation) and the sham operated group. GC-MS was used with pattern recognition technology and principal component analysis (PCA) to analyze the plasma samples at 4, 21 and 45 day after operation.ResultsIt was determined that the period from 7 to 28 days was the stable time window of ischemic heart failure with qi deficiency and blood stasis syndrome (QDBS), and the qi deficiency syndrome occurred at 1 to 4 days and 45 to 60 days after operation. The results exhibited 5 plasma metabolite changes in the same trend at 4 and 21 day after the LAD operation, 7 at 21 and 45 day, and 2 at 4 and 45 day. No metabolite showed the same change at all of the 3 time points. At day 21 (the QDBS syndrome time point) after operation, 4 plasma metabolites showed the same trends with the results of our previous study on patients with the blood stasis syndrome.ConclusionsThe syndrome diagnosis is reliable in the HF rat model in this study. Plasma metabolites can provide a basis for the evaluation of Chinese medicine syndrome animal models.

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“…So these differential metabolites can be considered as potential metabolic biomarkers of Qi deficiency and Blood Stasis Syndrome. Luo et al [17] studied the plasma metabolic phenotype in rats with Syndrome of Liver Qi Stagnation and spleen deficiency. Compared with control group, model rats displayed significant changes in spectral peak shapes of acetate, lactate, tyrosine, low-density lipoprotein.…”

Section: Applications Of Metabonomics In Tcmmentioning

confidence: 99%

The Applications and Obstacles of Metabonomics in Traditional Chinese Medicine

Liu

Tian

et al. 2012

Evidence-Based Complementary and Alternative Medicine

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In the recent years, a wide range of metabonomic technologies are widely used in the modern research of traditional chinese medicine (TCM). At present, the most prevailing methods for TCM research are mainly nuclear magnetic resonance (NMR), gas chromatography-mass spectrometry (GC-MS), and liquid chromatography-mass spectrometry (LC-MS). With these techniques, metabonomics will help to understand syndromes, efficacy and toxicity of TCM. However, every analytical technique has its advantages and drawbacks, and there exist some obstacles of its applications on TCM. So, we discuss metabonomics in TCM and analyze some problems of its applications to study TCM in recent years. We believe that with the further development of metabonomic analytical technology, especially multianalysed techniques, metabonomics will greatly promote TCM research and be beneficial to the modernization of TCM.

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Metabonomic study of syndrome of liver qi stagnation and spleen deficiency in rats

Cited by 19 publications

References 0 publications

Metabonomic phenotype and identification of “heart blood stasis obstruction pattern” and “qi and yin deficiency pattern” of myocardial ischemia rat models

Metabonomic phenotype and identification of “heart blood stasis obstruction pattern” and “qi and yin deficiency pattern” of myocardial ischemia rat models

Plasma metabonomics study on Chinese medicine syndrome evolution of heart failure rats caused by LAD ligation

The Applications and Obstacles of Metabonomics in Traditional Chinese Medicine

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