In this contribution, the conservation status assessment of two vascular plants according to IUCN categories and criteria is presented. It includes the regional assessment of Jasione orbiculata Griseb. ex Velen. for Italy and of Vachellia gerrardii (Benth.) P.J.H.Hurter subsp. negevensis (Zohary) Ragup., Seigler, Ebinger & Maslin for Iraq.

Dirofilaria immitis, the cause of heartworm infestation (HWI) or dirofilariasis, affects members of the Canidae and remains a worldwide clinical problem. In Iraq, dirofilariasis was believed absent until 2009, when the Karbala Governorate was reported as an endemic area for canine dirofilariasis. Consequently, this study intended to investigate the occurrence of Dirofilaria immitis in police dogs in one police academy in Iraq and to study the gross and histopathological changes in 5 dead dogs, as well as to identify the species of the causative parasite using PCR technique. Thirty-nine police dogs, aged between 6 months and 12 years were included in this study. For the microfilariae investigation, 5 ml blood samples were collected from all dogs in EDTA tubes and examined by Knott’s method. The systemic necropsy performed in five dead dogs showed severe clinical signs of dirofilariasis and tissue specimens were sent for routine histopathological processing. For the molecular analysis, adult worms of the detected Dirofilaria spp. were used for DNA extraction andamplification of the cox1 gene. Fifteen of 39 (38.46%) dogs were diagnosed with moderate to severe microfilariasis. The dead dogs revealed typical severe clinical signs of dirofilariasis. Moreover, typical gross and histopathological changes were also seen, accompanied by generalized thromboembolic lesions, suggesting the occurrence of the caval syndrome. The PCR investigation confirmed that D. immitis was the species present in Iraq. In conclusion, this study establishes that Iraq is a newly reported endemic area for dirofilariasis. Moreover, the infestation occurring in these cases most probably happened inside Iraq. The authors recommend doing further epidemiological studies concerning the occurrence of D. immitis in local dogs as well as in the imported dogs in all Iraqi governorates to better understand the epidemiological map of this disease and to introduce an active treatment and preventive program. Awareness and education regarding this disease should be provided to the veterinarians, dog guiders and people in direct contact with dogs, as this disease is one of the important zoonotic diseases.

One eighth of the bird species in the world is considered globally threatened; the avifauna of Iraq comprises 409 species and is considered as the major indicator of the health of Iraq’s biological resources. The Iraqi geography falls into five main regions among which is the desert and semi-desert areas which cover much of the country area. Al-Najaf desert is still one of the poorly known regions from the biodiversity point of view. Birds of conservation concern are detected in Al-Najaf desert during 31 field trips to 20 sites conducted from August 2018 to April 2020, (citing literature records, and personal interviews with locals).The factors caused the bird numbers to decline in Al-Najaf desert include hunting and trapping, logging, invasive species, and climate change. Nine birds are found threatened with 3 EN and 6 VU comprising Saker Falcon Falco cherrug Gray, 1834 (Falconidae, Falconiformes), Red-footed Falcon Falco vespertinus Linnaeus, 1766 (Falconidae, Falconiformes), Steppe Eagle Aquila nipalensis Hodgson, 1833 (Accipitridae, Accipitriformes), Egyptian Vulture Neophron percnopterus (Linnaeus, 1758) (Accipitridae, Accipitriformes), Greater Spotted Eagle Aquila clanga (Pallas, 1811) (Accipitridae, Accipitriformes), Marbled Teal Marmaronetta angustirostris (Ménétrés, 1832) (Anatidae, Anseriformes), Macqueen's Bustard Chlamydotis macqueeni (Gray, 1834) (Otididae, Otidiformes), Turtle Dove Streptopelia turtur (Linnaeus, 1758) (Columbidae, Columbiformes), and Southern Grey Shrike Lanius meridionalis aucheri Bonaparte, 1853 (Laniidae, Passeriformes). It is concluded that Al-Najaf desert is a region of top priority area for biodiversity conservation as it hosts large number of threatened bird species.

The research was carried out in Babylon province on 138 domestic and wild columbide birds were collected during the period from April to June 2012. Fresh saline smears samples (wet mount) were taken from mucosa of buccal cavity, pharynx ,esophagus and crop of each bird, then examined by wet mount looking for Trichomoniasis, then the intestine opened longitudinally examined looking for helminthes which examined grossly and after fixative staining with carmine stain for identification. The results of wet mount for Trichomonus technique revealed that the total rate of infection was 43(31.5%). Asignificant differences were recorded in the rates of infection regarding the species of birds. The highest rate of infection was recorded with Trichomonus gallinae, in wood pigeon 16 (53.33%) followed by Rock pigeon, Domestic pigeon, Collared dove and palm dove 12(40%), 9 (30%), 4(13.34%) and 2(11.12 %) respectively. The results revealed that the total rate of infection was 80 (57.97%), with cestodes parasite. Asignificant differences regarding the type of the birds, the highest prevalence was recorded in wood pigeons 27 (90%) followed by rock pigeon, collared dove and domestic pigeons in prevalence rate 25 (83.34%), 12 (40%) and16 (26.67%) respectively, while no cestods recorded in palm dove. Asignificant differences regarding the type of cestodes parasite. Out of 123 collected cestodes, 79 (64.23%) were geuns Raillietina (18.69%) were geuns Aporina, and (17.07%) were geuns Cotugnia

Many thousands of tree species are declining in the world, for which conservation actions are urgent. This dramatic situation is particularly evident for trees closely related to freshwater ecosystems, considered the environments most threatened by global change. In extremely arid environments such as deserts, where the few plant species present are able to survive by exploiting the little water available, the situation is extremely critical. A representative case is Vachellia gerrardii subsp. negevensis, a tree with a wide distribution range but locally restricted to small and isolated populations. Knowledge about this tree is incomplete in Iraq and, to fill this gap, several surveys were conducted in the Al-Najaf desert over three years (2019–2021). In each locality where this species was found, several ecological and population parameters were recorded. Our results indicate that Vachellia gerrardii subsp. negevensis was restricted to a single population, fragmented into five subpopulations confined in extremely peculiar ecological niches. Several threats related to anthropogenic activities and climate change affect the population, causing a significant reduction in the population size combined with a continuous decline in habitat quality and number of mature plants. Despite this critical situation, there are no conservation measures for this species. The establishment of a national preserve or part would be important and constitutes the fundamental prerequisite for the conservation of multiple species.

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In this contribution, the conservation status assessment of three vascular plants according to IUCN categories and criteria is presented. It includes the global assessment of Limonium parvifolium Tineo and Viscaria alpina (L.) G.Don, and the regional assessment of Rhazya stricta Decne. (Iraq).

This study was designed to detect equine piroplasmosis using the molecular technique in Al-Najaf province during the season that showed an increment in tick activities. Blood samples were collected from 110 horses with more than two signs of piroplasmosis. After DNA extraction, the product was examined by a polymerase chain reaction to amplify 18SrRNA. The results showed that the overall percentage of equine theileriosis was 38.18%. According to gender, the percentage of infection was 43.48% and 29.27% in females and males, respectively. Significant variations appeared between infected horses according to age, and the percentage of infection was 50% and 35.22% in less than 2 years and more than 2 years age, respectively. Moreover, the percentage of infection was 62.5% and 19.35% in animals with and without acariasis, respectively. Significant variations were also seen in equine theileriosis according to geographical areas, and the higher percentage was reported in Hera district (60.87%), while the lowest percentage was in the center of Al-Najaf (21.43%). This difference may be due to different distribution of vector of disease (tick), which may be the availability of the suitable weather that helped in the multiplication of the intermediate vectors. In conclusion, this study proved the variations in the occurrences of equine piroplasmosis according to gender, age, and geographical areas.