Design and characterization of novel stationary phases based on retention behavior studies with various aromatic compounds Characterization of stationary phases by retention behavior studies with aromatic compounds is reviewed, focusing mainly on the authors' approach. Various aromatic compounds, such as polycyclic aromatic hydrocarbons (PAHs) and fullerenes, of different molecular shape and size were employed as the sample probes. For the systematic analysis of retention behavior, some spectroscopic techniques and theoretical calculations, such as energy minimized calculation of the phase structure, have also been employed to confirm the results from these retention studies. During these investigations, microcolumn chromatography was employed in order to evaluate small amounts of experimentally synthesized bonded phases. The design of several novel stationary phases, based on the molecular shape recognition concept, is also briefly reviewed along with the applications.
The separation of fullerenes with monomeric and polymeric octadecylsilica (ODS) bonded phases has been studied and the existence of three C78 isomers confirmed. It has been found that the elution order of higher fullerenes is controlled by their molecular size and shape and that polymeric ODS resolves three C7g isomers, one being eluted faster than C76. Monomeric ODS cannot resolve two of the C7g isomers, although a lower column temperature of 10 °C gives its partial separation.UV-visible spectral assignments were confirmed by FAB-MS measurements of the fractionated samples.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.