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t Technical Services Section, Aedes aegypti Eradication Branch. VoL14. No. I Pr@@ntal i,@ U.S.A. surveyed in 1964. The states were divided into two groups based on previously known extent of infestation. In the group of states with extensive infestationsâ€"Florida, South Carolina, Georgia, Alabama, Mississippi, Louisiana, and east Texas â€"onlycounties with recent negative surveys were omitted. In the group of marginal states with little or no infestationâ€"NorthCarolina, Tennes see, Arkansas, and Oklahomaâ€"counties with largecities or locatednear known infestations were surveyed. In this manner 58% of the coun ties which contained 64% of the population in the area were surveyed. The survey consisted of inspection of premises most likely to be infested ineach community in the county. The size of the survey in a county differed, based on human population, urban na ture, and number of communities. The general requirement was to survey one percent of the premises in the county. In a few cases where the city being inspected was very large or where Aedes aeqypti infestation was found immediately, less than one percent of the premises were sur veyed.In most counties, surveyswere completed

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LABORATORY rearing of Aedes aegypti 1 (L.) has been a common procedure among entomologists and other scientists for about 50 years. Many improvements in equipment and techniques have been developed (1-3), and production by these methods has proved ade¬ quate for studies using as many as several thousand specimens. Experiments on dispersal (4) and on control by release of sexually sterile males (5, 6), however, require the sustained production of millions of specimens. This paper describes equipment and procedures de¬ veloped during 1955 and 1956 for rearing broods of as many as 1.3 million larvae. Procedures Maintenance of adult colonies. The strain of A. aegypti used in the studies had been main¬ tained in the Technical Development Labora¬ tories of the Communicable Disease Center in Savannah, Ga., since 1944. Two laboratory rooms, each 10 by 13 by 10 feet and maintained at about 80°F. and 80 percent relative humi¬ dity, were required for the mass production of A. aegypti. One room was used for adult colonies, emergence cages, and egg storage. The second room contained the larval rearing trays.

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