The physiological character, especially the effect of pH and organic substrate on the growth and activity of some ammoniaoxidizing bacteria was carried out. The results show that eight out of twenty isolates have ability to reduce ammonium, two of them i.e. isolate AOB1 and AOB2 could reduce more than 90% of ammonium. The growth and activity to reduce ammonium to nitrite was attained optimum at pH 7-8. From the result also indicated that the growth and activity of both isolate AOB1 and AOB2 were higher on the organic carbon (acetate)-containing media. This finding indicated that both of isolate AOB1 and AOB2 were heterotrophic ammonia-oxidizing bacteria.
Nitrification is often not effective because the characters of nitrifying bacteria is always slow growing and temperature, pH, DO, amonia concentration, nitrite and C/N ratio as the limited factors. Batch experiment was operated for 6 hours, every half an hours ammonium, nitrite and nitrate were measured.The results showed that added C sourse (glucose) in medium from PDAM Bogorand Surabaya have a significant effects of ammonium degradation, nitrite and nitrate production. Degradation of ammonium in batch reactor from PDAM Bogor showed that both naerobic and aerobic condition have a different fluctuation. Production of nitrite was stable from the middle of reaction to last of aerobic phase. In aerobic condition nitrate production is reduced to 49,21 % (+ glucose) and 60,87% (+ acetate) Batch reactor from PDAM Surabaya differ from PDAM Bogor particularly on degradation of ammonium. Added glucose as C source cause the concentration of ammonium was increased and acetate as C source showed stable with a slightly fluctuation. Nitrite and nitrate production relatively small in both C source.
Propoxur (2-isopropoxyphenyl-N-methylcarbamate) is a carbamateinsecticide which has negative impact on the environment. Propoxur is readily degraded by soil microorganism in most soils. Increased enviromental degradation of propoxur constitute the result of increase in activities of soil pesticide-degrading bacteria. The aims of the present study are to isolate andscreen the bacteria with high potential ability to degradepropoxur from soil samples. Isolation and selection were carried out by enrichment cultures technique.Propoxur degrading bacteria were isolated from 5 soil samples in different locations of rice field from Ngawi. The biodegradation ofpropoxur by isolated bacteria were analysed by High Performance Liquid Chromatography (HPLC). Ten consorsia were selected and purified. One isolate was able to use propoxur at 3000 ppm as sole source of carbonfor the growth. The result suggested that H2-NG isolate hydrolysedpropoxur with the yield of 2-isopropoxyphenol and methylamine.
Soil pollution has been so attracting considerable public attentions over the last decades. Phytoremediation is an emerging technology that uses plants to clean up pollutant soils. The study was carried out in gold mining Cikotok, Banten. Sengon buto (Enterolobium cyclocarpum, (Willd) which is used as cyanogenic plant, compost and microbes inoculant is as stimulator to growth this plant. The experiment consist of K0 as control (plant without compost), K1 as plant + compost and K2 as plant+compost + microbe inoculant, with 3 replicated. The objectives of this experiment were to investigate the potential for phytoremediation of cyanide contaminated soils using hyperaccumulator/cyanogenic plants and to assess the fate and transport of cyanide compounds in soils. The results showed that compost and microbe were able to stimulate growth of Sengon Buto after 7 months planting and to reduce cyanide until 66% Total bacteria in the study was relatively stable but NFB bacteria was decline.
This research investigate the population and potentials denitrifi cation activity from three different soils, organically farmed soil, intensive farmed soil and forest soil. Our objectives were to explore spatial gradients in denitrifi er populations, examine whether populations density and its potential activity was related to soil chemical properties (C and N content), and determine the potential emission of gas N 2 O. Results indicated biological functional differences between these three different soil ecosystems. Forest soil had the highest population density of denitrifying bacteria and also had signifi cant potential denitrifying activities. The highest potentials denitrifying activity in the soil affected to the lowest emission of N 2 O gas. The lowest population and potential denitrifying activity was measured in the intensive farmed soil. Those conditions might be promoted the potentials emission of N 2 O.
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