Effect of pH and organic substrate on growth and activities of ammonia-oxidizing bacteria

Agustiyani, Dwi; Imamuddin, Hartati; Faridah, Erni Nur; Oedjijono, Oedjijono

doi:10.13057/biodiv/d050201

Cited by 10 publications

(6 citation statements)

References 1 publication

(1 reference statement)

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“…However, the present study showed that biochar suppressed amoA-AOB and amoA-AOA gene abundance. The explanation was that biochar addition decreased the frequency of functional microorganisms by increasing the diversity of AOB and AOA, and increased soil pH (Table 1), which exceeded the optimum value for amoA growth and activity [50].…”

Section: Biochar Addition Shifted the Community Structures Of Aob And...mentioning

confidence: 99%

Biochar Addition Inhibits Nitrification by Shifting Community Structure of Ammonia-Oxidizing Microorganisms in Salt-Affected Irrigation-Silting Soil

Yao

Yang

et al. 2022

Microorganisms

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Biochar has been widely recognized as an effective and eco-friendly ameliorant for saline soils, but information about the mechanism of how biochar influences nitrification in salt-affected agroecosystem remains fragmented. An incubation experiment was performed on the salt-affected soil collected from a three-consecutive-year experiment at biochar application gradients of 7.5 t⋅ha−1, 15 t⋅ha−1 and 30⋅t ha−1 and under nitrogen (N) fertilization. Responses of the nitrification rate (NR), numbers of ammonia monooxygenase (amoA) gene copies, and community structures of ammonia-oxidizing bacteria (AOB) and archaea (AOA) to biochar application were investigated. The results indicated that, under N fertilization, the NR and numbers of amoA-AOB and amoA-AOA gene copies negatively responded to biochar addition. Biochar application increased the community diversity of AOB but decreased that of AOA. Biochar addition and N fertilization shifted the AOB community from Nitrosospira-dominated to Nitrosospira and Nitrosomonas-dominated, and altered the AOA community from Nitrososphaera-dominated to Nitrososphaera and Nitrosopumilus-dominated. The relative abundance of Nitrosospira, Nitrosomonas and Nitrosopumilus decreased, and that of Nitrosovibrio and Nitrososphaera increased with biochar application rate. Soil SOC, pH and NO3−-N explained 87.1% of the variation in the AOB community, and 78.1% of the variation in the AOA community was explanatory by soil pH and SOC. The SOC and NO3−-N influenced NR through Nitrosovibrio, Nitrosomonas, Norank_c_environmental_samples_p_Crenarchaeota and amoA-AOB and amoA-AOA gene abundance. Therefore, biochar addition inhibited nitrification in salt-affected irrigation-silting soil by shifting the community structures of AOB and AOA and reducing the relative abundance of dominant functional ammonia-oxidizers, such as Nitrosospira, Nitrosomonas and Nitrosopumilus.

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Section: Biochar Addition Shifted the Community Structures Of Aob And...mentioning

confidence: 99%

Biochar Addition Inhibits Nitrification by Shifting Community Structure of Ammonia-Oxidizing Microorganisms in Salt-Affected Irrigation-Silting Soil

Yao

Yang

et al. 2022

Microorganisms

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“…Soil media was prepared by enriching the sieved soil material with a nitrification medium to stimulate nitrification activity and the growth nitrifier community. A liter of nitrification medium contained 0, 200, or 500 mg (NH4)2SO4; 725 mg KH2PO4; 1136 mg Na2HPO4; 50 mg MgSO4•7H2O; 20 mg CaCl2•2H2O; 1 mg Fe.EDTA; and 1 mL trace elements (Agustiyani et al 2004). The trace elements were made by dissolving 10 mg Na2MoO4•2H2O, 20 mg MnCl2•4H2O, 10 mg ZnSO4•7H2O, 0.2 mg CoCl2•6H2O, and 2 mg CuSO4•5H2O in 1000 mL aquabides (Agustiyani et al 2004).…”

Section: Preparation Of Soil Media and Nitrification Mediummentioning

confidence: 99%

“…A liter of nitrification medium contained 0, 200, or 500 mg (NH4)2SO4; 725 mg KH2PO4; 1136 mg Na2HPO4; 50 mg MgSO4•7H2O; 20 mg CaCl2•2H2O; 1 mg Fe.EDTA; and 1 mL trace elements (Agustiyani et al 2004). The trace elements were made by dissolving 10 mg Na2MoO4•2H2O, 20 mg MnCl2•4H2O, 10 mg ZnSO4•7H2O, 0.2 mg CoCl2•6H2O, and 2 mg CuSO4•5H2O in 1000 mL aquabides (Agustiyani et al 2004). All of the nitrification medium ingredients were dissolved in 1000 mL of distilled water and were sterilized at 121°C for 20 minutes.…”

Section: Preparation Of Soil Media and Nitrification Mediummentioning

confidence: 99%

Ammonium enrichment reduces the diversity and changes the composition of ammonia-oxidizing microbial communities in agricultural soil media

SAUKOLY,

MEITINIARTI,

NUGROHO

et al. 2024

Biodiversitas

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Abstract. Saukoly SA, Meitiniarti VI, Nugroho RA, Krave AS. 2024. Ammonium enrichment reduces the diversity and changes the composition of ammonia-oxidizing microbial communities in agricultural soil media. Biodiversitas 25: 916-923. Nitrification is the process of ammonium oxidation to form nitrites and nitrates. Ammonium availability in the soil is one of the factors influencing the activity, abundance, and diversity of ammonia-oxidizing microorganisms (AOM). This study aimed to determine the effect of enriching a soil media with different ammonium concentrations on the abundance and diversity of AOM as well as the potential for nitrification. Soil as the media was prepared from an agricultural land receiving cow dung sewage. The media was then placed in the microcosms, and was added to a nitrification medium containing ammonium at three levels; 0 (control soil), 200 (low ammonium-enriched soil), and 500 mg L-1 (high ammonium-enriched soil). The microcosms were further incubated for 42 days at room temperature. The nitrification potential determination was based on the formation of nitrite from ammonium oxidation. The abundance of AOM and the potential nitrification were measured every 14-day interval, including day 0 as the initial condition. The AOM composition was analyzed based on the similarity level of the amoA gene sequence to the NCBI BLAST GenBank database. However, this analysis was only conducted for the control and high ammonium-enriched soil. This study indicated that ammonium enrichment increased the nitrification activity and the abundance of AOM, but it decreased the diversity of AOM communities. There were positive correlations between nitrification and nitrate potential, as well as between ammonium content and AOM abundance. Negative correlations appeared between pH and nitrification potential, nitrate concentrations, ammonium concentrations, and MPN. The diversity of ammonium-oxidizing archaea (AOA) in the control soil was higher than in the high ammonium-enriched soil. The enrichment with ammonium also changed the AOA composition. The Candidatus Nitrosocosmicus oleophilus strain of the MY3 chromosome was the most dominant archaea in the control and high ammonium-enriched soil. This implies that the high diversity of AOA in this soil may be beneficial for further use of the soil as a source for inocula in the development of nitrifiers-based biofertilizers.

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“…Therefor Agrobacterium sp I37 added to the soil increased total microbial colonies in the soil. The more total microbial colonies in the soil, the greater the likelihood of interaction between microorganisms and root exudates, thereby enhancing the ability of plants to absorb heavy metals (Gadd, 2010;Agustiyani et al, 2004). The use of compost and inorganic fertilizers can supply C-organic in the soil thereby increasing the population of microorganisms (Zhang et al, 2015).…”

Section: Total Microbial Coloniesmentioning

confidence: 99%

Effort to Get Safe Rice for Consumption Through Bioremediation Technology in Paddy Field Contaminated by Lead

Johanto

Rosariastuti

Cahyani

2019

Trop Subtrop Agroecosyst

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Textile industry that has less optimal waste management due to the absence of a wastewater treatment plant (WWTP) tends to dispose the waste directly into the water or river. Most farmers near industrial area use liquid waste for irrigation water. This generates negative impacts of complicated problems in the agricultural sector, especially heavy metals contamination in paddy soils. In such case, bioremediation can be done to handle the contaminated soils by heavy metals to create a healthy farming environment. The purpose of this study was to determine the effectiveness of the chelating agents (Agrobacterium sp. I37 or compost) to the characteristic of soil and the level of Pb in rice plant. This study was conducted between June - September 2017 in Tasikmadu, Karanganyar, Indonesia. The experimental design was factorial using randomized completely block design (RCBD) as the based design. There are two factors in this research: a) inorganic fertilizer (P): without inorganic fertilizer (P0) with inorganic fertilizer (P1); b) chelator (B): without chelator (B0), with chelator Agrobacterium sp I37 (B1), with chelator compost (B2). There were six treatment combinations then replicated 3 times for each treatment, so there were 18 unit treatments. The results showed that Agrobacterium sp. I37 or compost can decrease soil Pb. The best treatment in decreasing soil Pb was treatment using Agrobacterium sp. I37, which soil Pb decreased of 42.91% from 8.04 mg/kgto 4.59 mg/kg; could enhance accumulation of Pb in the roots of 69, 63% and decrease Pb levels in rice grains around 55.67% higher than control treatment.

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Effect of pH and organic substrate on growth and activities of ammonia-oxidizing bacteria

Cited by 10 publications

References 1 publication

Biochar Addition Inhibits Nitrification by Shifting Community Structure of Ammonia-Oxidizing Microorganisms in Salt-Affected Irrigation-Silting Soil

Biochar Addition Inhibits Nitrification by Shifting Community Structure of Ammonia-Oxidizing Microorganisms in Salt-Affected Irrigation-Silting Soil

Ammonium enrichment reduces the diversity and changes the composition of ammonia-oxidizing microbial communities in agricultural soil media

Effort to Get Safe Rice for Consumption Through Bioremediation Technology in Paddy Field Contaminated by Lead

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