The synthesis, antimicrobial, and antioxidant activities of new quinoline analogs were carried out with the aim to find possible hits/leads that can be taken up for future drug development. A series of 2-amino-N’-((2-chloroquinolin-3-yl)methylene)acetohydrazide derivatives (6a-h) have been synthesized by reacting 2-chloro-N’-((2-chloroquinolin-3-yl)methylene)acetohydrazide (5a) and N’-((6-bromo-2-chloroquinolin-3-yl)methylene)-2-chloroacetohydrazide (5b) with secondary amines (Morpholine, diethylamine, piperidine and 1-methylpiperazine). The characterization was achieved by FT-IR, 1H NMR, 13C NMR, and mass spectral analysis. The in silico ADMET studies of the synthesized molecules were analyzed for their drug likeliness and toxic properties. The ADMET study indicates that the synthesized compounds were found to be possessing reliable ADME properties and are nontoxic. The antimicrobial properties were tested against bacterial and fungal species with amoxicillin and fluconazole as standard drugs. The compounds 6a, 6c, 6e, and 6g exhibited good antibacterial potency against P. aeruginosa, and the compounds 6a, 6f, and 6h have shown good activity against E. coli with 1000 µg/mL. The compounds 6b, 6c, and 6e have moderate activity against fungal species C. oxysporum and the compounds 6c, 6e, 6f, 6g, and 6h have good activity against P. chrysogenum. Synthesized compounds were also tested for the DPPH· free radical scavenging activity to check the antioxidant potential, and the results revealed that the compounds 6a, 6b, 6c, and 6e have exhibited antioxidant potency than the remaining synthesized derivatives. The possible hits generated from biological activity could be taken for the generation of lead molecules for the drug discovery of antimicrobial and antioxidant entities from quinoline.

An increase in free radical concentration in the human body due to medications leads to oxidative stress can be counteracted by novel antioxidative agents that lower the concentration of free radical and free radical damages in human body. In present study, 2-thiophene 4-thiadiazole quinoline derivatives (5a-e) were designed and synthesized using 2-thiophene quinoline 4-carboxylic acids and thiosemicarbazide. The designed compounds 5a-e were docked against the protein PDB-ID: 1OC3 and evaluated the antioxidant activity using DPPH assay and also screened for antibacterial and antifungal potential by Agar well diffusion assay followed by in vitro antitubercular assay by MABA method. The IC50 values for compounds 5a and 5b were 415 μg/mL and 396 μg/mL. The binding affinity of docked ligands against the protein 1OC3 ranges from -6.2 to -5.7 kcal/mol. In an antimicrobial investigation, the compounds were found to be active against both bacteria and fungi, as well as sensitive to M. tuberculosis.