Cell culture and cell scaffold engineering have previously developed in two directions. First can be ‘static into dynamic’, with proven effects that dynamic cultures have benefits over static ones. Researches in this direction have used several mechanical means, like external vibrators or shakers, to approximate the dynamic environments in real tissue, though such approaches could only partly address the issue. Second, can be ‘2D into 3D’, that is, artificially created three-dimensional (3D) passive (also called ‘static’) scaffolds have been utilized for 3D cell culture, helping external culturing conditions mimic real tissue 3D environments in a better way as compared with traditional two-dimensional (2D) culturing. In terms of the fabrication of 3D scaffolds, 3D printing (3DP) has witnessed its high popularity in recent years with ascending applicability, and this tendency might continue to grow along with the rapid development in scaffold engineering. In this review, we first introduce cell culturing, then focus 3D cell culture scaffold, vibration stimulation for dynamic culture, and 3DP technologies fabricating 3D scaffold. Potential interconnection of these realms will be analyzed, as well as the limitations of current 3D scaffold and vibration mechanisms. In the recommendation part, further discussion on future scaffold engineering regarding 3D vibratory scaffold will be addressed, indicating 3DP as a positive bridging technology for future scaffold with integrated and localized vibratory functions.
The categorization of cell culture chiefly includes two aspects; one is the dimensionality and another regards the dynamicity. Referring to knowledge of "engineering system evolution", 2D toward 3D cell culture follows the direction of evolution in dimensionality, and 3D scaffolds with 3DP as its popular fabrication tools has played a role in 3D cell culture applications. Dynamic methods of cell culturing, compared with traditional static means, generally follow the evolution line "static to motional or dynamic", and vibration has been selected frequently as the suitable tool to achieve the dynamicity of cell culture. Although such a scaffold plus vibration approach has benefited cell culture, there exist significant defects. To mitigate some existing gaps, as well as following further evolutionary trends, the concept of the 3D printed vibratory scaffold (3DPVS) used in cell culture applications is firstly brought out in this study. With 3DPVS, a 3D scaffold in traditional scaffold engineering could potentially evolve into a novel vibratory scaffold which will play significant role in future bioengineering and scaffold engineering. Since 3DPVS's development remains blank, designers firstly need to propose a high-quality conceptual design; the process of identifying design methodology is challenging since there has been no formal methodology applied for scaffold design. To address these issues, a new design approach is proposed in this paper, which includes an integral development process and focuses on the 3DPVS conceptual stage. The possible methodology and tools to achieve the established conceptual design in following step will be also be discussed.
Research related with scaffold engineering tends to be cross-domain and miscellaneous. Several realms may need to be focused simultaneously, including biomedicine for cell culture and 3D scaffold, physics for dynamics, manufacturing for technologies like 3D printing, chemistry for material composition, as well as architecture for scaffold’s geometric control. As a result, researchers with different backgrounds sometimes could have different understanding towards the product described as ‘Scaffold’. After reviewing the literature, numerous studies termed their developed scaffold as ‘novel’, compared with scaffolds previously designed by others using comparing criterion like ‘research time’, ‘manufacturing method’, ‘geometry’, and so on. While it may have been convenient a decade ago to, for example, categorize scaffold with ‘Dualistic Thinking’ logic into ‘simple-complicated’ or ‘traditional-novel’, this method for categorizing ‘novelty’ and distinguishing scaffold is insufficiently persuasive and precise when it comes to modern or future scaffold. From this departure of philosophical language, namely the language of ‘relativity’, it is important to distinguish between different scaffolds. Other than attempting to avoid ambiguity in perceiving scaffold, this language also provides clarity regarding the ‘evolution stage’ where the focused scaffolds currently stand, where they have been developed, and where in future they could possibly evolve.
An important product in biomedical and biomimetic engineering is the 3D scaffold, which mimics the real tissue in vitro to achieve the external cultivation of cells. The difference between the 3D scaffold and other biomimetic products lies in the fact that the former mimics the internal features of tissue, while the latter generally approximates the external traits of biological beings. In the field of scaffold engineering, the 3D printed vibratory scaffold, 3DPVS, has been proposed as a present-to-future novel scaffold product, and it currently stays at the stage of conceptual development. To achieve the novel design of the conceptual 3DPVS, a conceptual design process has been established by authors in their previous work, which contain three main stages, namely the design initiation, concept generation, and concept evaluation. In terms of design initiation, it is a ‘must-accomplish’ stage which generates outputs for both the subsequent concept generation and evaluation. Work of design initiation therefore is of significant value and it consists of several tasks; that is, conducting a thorough literature review, summarizing the fundamental issues preparing the general conceptual design, studying the multi-characterization of the 3DPVS, putting forward the potential base model(s), as well as indicating the ideality of the scaffold and establishing potential ideal model(s) for the 3DPVS. In this paper, design initiation will be chiefly focused upon these essential aspects to be discussed, work of which is expected to be useful in establishing a solid ground for future innovation work of the 3DPVS.
A fluorescence device based on ultraviolet light is proposed in this paper, which currently stands at the design stage with the eventual aim to potentially detect virus/antibody fluorescence reactions. The designed device is proposed to have the characteristics of high reflectivity, low power consumption, wide spectrum of light source, and proper silver coating. For fabrication and raising product quality, 3D printing technology and a sputtering test will be used. In this connection, this paper firstly introduces the design sources; then, the ideas of inventing fluorescence detection devices based on ultraviolet light, followed by the data analysis as well as discussing the results of computer simulations. The design process, materials, methods, and experiments are demonstrated following the reality work procedure. Instead of directly using a virus or antibodies for the experiment, at the current design stage, we focus on using this device to detect the rhodamine B reagent. Experiment shows that this reagent can be successfully detected. With this achievement, we logically believe that such type of an ultraviolet optical sensor, with further development and testing, may have the possible value to detect a single virus such as COVID-19, as well as other viruses or small molecules. Though there is long way to go to achieve such a goal, future works experimenting with the detection device on real virus or antibodies can take place more efficiently with a good foundation.
Underwater target localization is the most crucial part of the underwater wireless sensor network (UWSN). Due to limited communication range and energy constraints in underwater scenarios, only a subset of sensors can be selected to localize. This paper investigates the sensor selection schemes for hybrid angle-of-arrival (AOA) and time-of-arrival (TOA) localization in the underwater scenario. We first develop the Cramér-Rao lower bound (CRLB) for the hybrid AOA-TOA localization with correlated measurement noise model with Gaussian priors, and a Boolean vector is introduced to denote the selected sensors for hybrid measurement. Secondly, the sensor selection schemes are formulated as an optimization problem, and the optimality criterion is to minimize the trace of CRLB. The original nonconvex problem has been modified to the semidefinite problem program (SDP) by convex relaxation, and then, a randomization algorithm is chosen to advance the result of the SDP method. Finally, simulations verify that the proposed algorithm approaches the exhaustive search algorithm, and the effect of correlated measurement noise on the estimation performance in the hybrid localization system is proved.
Regarding the innovation of biomimetic cell culture scaffold, 3DPVS, namely 3D printed vibratory scaffold, has been proposed as a present-to-future novel product. It currently stands at the stage of conceptual development. Design studies on 3DPVS Concept Generation show high value, and one essential part inside this could dwell at establishing design methodological knowledge that has innovation merits. TRIZ with its tools has proven value on creation and design innovativeness while they have not yet been utilized for scaffold design at mature level. In this paper, we attempt to study and explore the design aspects of TRIZ and its most relevant tools on the context of 3DPVS, as well as preliminarily indicating a TRIZ-based methodology, which could tailor the design aspects of 3DPVS. It also, to some extent, fills a gap in scaffold engineering and TRIZ literature and provides a comprehensive overview of a timely topic.
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