In this study, eight subjects were exposed in a simulated office to 31 combinations of indoor environmental conditions, assigned by orthogonal design and uniform design. Conditions comprised variations of Predicted Mean Vote (PMV), illuminance, sound pressure and CO2 concentration (independent of a consistent ventilation rate) as indicators of thermal, lighting, acoustic and indoor air quality. Participant satisfaction with each of the four factors and with overall environmental conditions were measured with a questionnaire. Multiple interactions were detected with a partial correlation analysis and regression analysis.Results showed an adjusted effect of illuminance on perceived acoustic environment, a significant effect of the thermal environment on indoor air quality satisfaction, and a slight effect of sound pressure on indoor air quality satisfaction. Linear and geometric mean regression models were investigated for predicting overall satisfaction from the factor satisfaction scores. For the linear model, it was determined that multicollinearity among factor satisfaction levels may result in non-significant and biased estimated coefficients. The geometric mean regression model provides better prediction accuracy than the linear regression model with fewer coefficients, and accounts for the finding that the lowest satisfaction level with any environmental factor appears to drive overall satisfaction.
Little information is available regarding the effect of melatonin on the quality and fertilization capability of sex-sorted bull sperm, and even less about the associated mechanism. Sex-sorted sperm from three individual bulls were washed twice in wash medium and incubated in a fertilization medium for 1.5 h, and each was supplemented with melatonin (0, 10−3 M, 10−5 M, 10−7 M, and 10−9 M). The reactive oxygen species (ROS) and endogenous antioxidant activity (glutathione peroxidase (GPx); superoxide dismutase (SOD); catalase (CAT)), apoptosis (phosphatidylserine [PS] externalization; mitochondrial membrane potential (Δψm)), acrosomal integrity events (malondialdehyde (MDA) level; acrosomal integrity), capacitation (calcium ion [Ca2+]i level; cyclic adenosine monophosphate (cAMP); capacitation level), and fertilization ability of the sperm were assessed. Melatonin receptor 1 (MT1) and 2 (MT2) expression were examined to investigate the involvement of melatonin receptors on sex-sorted bull sperm capacitation. Our results show that treatment with 10−5 M melatonin significantly decreased the ROS level and increased the GPx, SOD, and CAT activities of sex-sorted bull sperm, which inhibited PS externalization and MDA levels, and improved Δψm, acrosomal integrity, and fertilization ability. Further experiments showed that melatonin regulates sperm capacitation via MT1. These findings contribute to improving the fertilization capacity of sex-sorted bull sperm and exploring the associated mechanism.
Venting range hoods can control indoor air pollutants emitted during residential cooktop and oven cooking. To quantify their potential benefits, it is important to know how frequently and under what conditions range hoods are operated during cooking. We analyzed data from 54 single family houses and 17 low-income apartments in California in which cooking activities, range hood use, and fine particulate matter (PM2.5) were monitored for one week per home. Range hoods were used for 36% of cooking events in houses and 28% in apartments. The frequency of hood use increased with cooking frequency across homes. In both houses and apartments, the likelihood of hood use during a cooking event increased with the duration of cooktop burner use, but not with the duration of oven use. Actual hood use rates were higher in the homes of participants who self-reported more frequent use in a pre-study survey, but actual use was far lower than self-reported frequency. Residents in single family houses used range hoods more often when cooking caused a discernible increase in PM2.5. In apartments, residents used their range hood more often only when high concentrations of PM2.5 were generated during cooking.
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