Background
DNA-binding one zinc finger (Dof) proteins are plant-specific transcription factors important for seed development, hormone regulation, and defense against abiotic stress. Although drought stress is a key determinant of plant physiology and metabolic homeostasis, the role of Dof genes in different degrees of PEG6000-induced drought stress has received little attention.
Methods
Tea plants (Camellia sinensis) were exposed to mild, moderate and severe drought stress. The Tea Genome and Plant TFDB databases were used to identify Dof gene family members in the tea plant. Clustal W2.1, MEGA6.0, ScanProsite, SMART, ExPASy, GSDS, MEME and STRING were used to build a phylogenetic tree, predict the molecular masses and isoelectric points of the Dof proteins, and construct a predicted protein-protein interaction network between the CsDof TFs and proteins in the A. thaliana database. The expression patterns of Dof genes in different tissues were analyzed, and qRT-PCR was used to measure the expression of Dof genes under different degrees of drought stress in tea.
Results
We identified 16 Dof genes in tea (C. sinensis cv. Huangjinya) using whole-genome analysis. Through comparative analysis of tea and Arabidopsis thaliana, we divided the Dof genes into four families (A, B, C, and D). We identified 15 motifs in the amino acid sequences of the CsDof proteins. Gene sequences and motif structures were highly conserved among families, especially in the B1 and C2 subfamilies. The protein-protein interaction network indicated that multiple CsDof proteins may be involved in the response to drought stress. Real-time PCR was used to examine the tissue-specific expression patterns of the CsDof genes and to measure their responses to different levels of PEG6000-induced drought stress in mature leaves. Most CsDof genes responded to drought stress. These results provide information on the Dof gene family in tea, offer new insights into the function of CsDof genes in a perennial species, and lay the foundation for further analysis of their functions.
The mirid bugs are one of the most important piercing–sucking insect pests in tea plantations, which severely reduce the quality and economic benefits of tea. In this study, the mirid bug species in the three tea-producing areas in Shandong Province of China were investigated. The distribution and occurrence of dominant species of mirid bugs on four weed host plants and tea plants Camellia sinensis (L.) O. Kuntze (Theaceae) were also studied in the tea agro-ecosystems. The results showed that Apolygus lucorum (Meyer-Dür) (Hemiptera: Miridae) was the dominant mirid bug species in the tea growing areas. Apolygus lucorum densities on Humulus scandens (Lour.) (Moraceae) and Artemisia lavandulaefolia DC. (Asteraceae) were relatively higher than those on Conyza canadensis (Linn) Cronq (Asteraceae), Artemisia annua Linn (Asteraceae), and C. sinensis. Host plant switching of A. lucorum in the tea agro-ecosystem was: A. lucorum scattered on and seriously infested tea plants in June and July; A. lucorum largely migrated to and gathered on H. scandens, A. lavandulaefolia, C. canadensis, and A. annua at the flowering stage, and population densities of A. lucorum on these flowering hosts peaked in late September; in October, A. lucorum gradually moved back to flowering tea plants. These results could provide a reference for selecting host plants, such as Artemisia plants, as trap plants for sustainable control of mirid bugs in tea plantations.
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