RAPD-PCR was employed for the characterization of S. aureus isolates from different sources such as soil, water, milk, meat and skin swab etc.,. Isolated pure cultures of S.aureus strains were subjected to genomic DNA isolation, purification, separation and quantification. Isolated DNA samples were distinguished by using 4 different random primers. Genome profile analysis obtained from the S.aureus demonstrated that it was possible to differentiate the S. aureus strains from different sources by RAPD technique. Results indicate possible relationships between host origin and genetic variation among S. aureus isolates from various sources. This RAPD method was thus useful for epidemiological studies of the S. aureus flora.
The guinea pig is one of the most relevant small animal models for studying a large number of infectious and non-infectious diseases. The major roadblock for its effective utilization is the lack of readily available molecular and immunological reagents. In order to address this issue, some of the important recombinant proteins such as IL-4, IL-10, IL-1β, MCP-1, IFN-y and TNFα which were generated previously were analyzed for their biological activity in terms of nitrite production in RAW cells. Interestingly, nitrite production was comparatively more in the presence of TNF-α in comparison with proinflammatory cytokine IFN-y and similar levels of nitrite production were observed in the presence of Th2 cytokines i.e IL-4 and IL-10. We have analyzed the biological activities of these recombinant proteins so that they could be used for a wide array of in vitro and in vivo biological assays.
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