Prokaryotic Expression, In Vitro Biological Analysis, and In Silico Structural Evaluation of Guinea Pig IL-4

Omanakuttan, Madhavan; Konatham, Hanumohan R.; Dirisala, Vijaya R.; Jeevan, Amminikutty; Mawatwal, Shradha; Dhiman, Rohan; Ly, Lan H.; McMurray, David N.

doi:10.1007/s12033-019-00227-w

Cited by 2 publications

(2 citation statements)

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“…(Figure 1). When anti-rgpIL-4 was added to the cells pretreated with IL-4, the production of NO was enhanced there by reversing the effect of IL-4 which is consistent with that of our previous report (Dirisala et al, 2019). It would be interesting to check the nitrite production in the presence of recombinant proteins generated from the variants generated by alternative splicing of genes.…”

Section: Biological Activities Of Recombinant Proteinssupporting

confidence: 89%

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Deciphering the Biological Activity of Recombinant Guinea Pig Proteins

Omanakuttan¹,

Dirisala²,

Konatham³

et al. 2020

CTBP

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The guinea pig is one of the most relevant small animal models for studying a large number of infectious and non-infectious diseases. The major roadblock for its effective utilization is the lack of readily available molecular and immunological reagents. In order to address this issue, some of the important recombinant proteins such as IL-4, IL-10, IL-1β, MCP-1, IFN-y and TNFα which were generated previously were analyzed for their biological activity in terms of nitrite production in RAW cells. Interestingly, nitrite production was comparatively more in the presence of TNF-α in comparison with proinflammatory cytokine IFN-y and similar levels of nitrite production were observed in the presence of Th2 cytokines i.e IL-4 and IL-10. We have analyzed the biological activities of these recombinant proteins so that they could be used for a wide array of in vitro and in vivo biological assays.

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Section: Biological Activities Of Recombinant Proteinssupporting

confidence: 89%

“…One of the major roadblocks of effective utilization of this animal model is the lack of readily available immunological reagents (3). Towards this initiative, coding sequences of genes pertaining to IL-4, IL-10, IL-1β, MCP-1, IFN-y and TNF-α were cloned and expressed for the generation of recombinant proteins (4,5,6,7,8,9).…”

Section: Introductionmentioning

confidence: 99%