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Two commercially available tests for the determination of LDL cholesterol were compared. The determination principle lies in the precipitation of LDL using heparin (Merck, Darmstadt test) or using polycyclic surface activated anions (bioMerieux test), äs well äs ascertainirig LDL cholesterol by subtracting cholesterol in the supernatant from total cholesterol (Merck, Darmstadt test) or the direct determination of cholesterol in the redissolved precipitate (bioMerieux test).

The use of platelet-rich plasma and growth factors is emerging as an anti-ageing regimen for the skin. We tested the safety and efficacy of 3D-MatrixLift®, a new treatment regimen for skin rejuvenation that combines medical needling and the application of a stem cell and growth factor-rich solution with irradiation by LED light. A total of 15 participants were enrolled in a single-centre, prospective pilot study. The elasticity parameters of the skin increased significantly after five rounds of treatment, with no signs of adverse effects. 3D-MatrixLift improves the elasticity of the skin and can be used safely in combination with medical needling for skin rejuvenation.

. Bilirubin increases latencies and reduces amplitudes in the auditory brainstem response (ABR). However, the basic mechanisms involved sre not known. The aim of the present study was to investigate the neurotoxic effects of bilirubin on synaptic transmission in an in vitro system. Hippocampal slices were prepared from 5-7 weeks old male Sprague-Dawley rats and incubated at 30-33'~ in an artificial cerebrospinal fluid equilibrated with 95% 0 and 5% CO to pH 7.4. We stimulated the Schaffer collaterals of th2 CA3 cells? Recordings were trade 3f the amplitudes of the presynaptic fibre volley (PV) and the field sxcitatory postsynaptic potential (EPSP) in the apical dendritic layer 3f the CAI region, and of the population spike (PS) in the corresponding cell body layer. The slices were exposed to bilirubin at >oncentrations up to ImM, in an 8:l molar ratio with bovine serum albumin (BSA). Over periods of 30-120 minutes a gradual reduction in the field EPSP amplitude was noted. In parallel with this the peak Latency of the PS increased. The stimulusfresponse relationships were sxamined with stimulus voltage from 1.0 to 3.4V. Bilirubin caused the 5VfEPSP curve to shift to the right, while the EPSPfPS curve shifted to the left. These changes were reversed when bilirubin was removed 2rom the perfusion fluid. The effect of bilirubin on rat hippocampal slices consists of a gradual and reversible decrease in synaptic transmission. This is consistent with the findings reported from ABR studies. The first child of consanguineous parents died at the age of 6 months i n renal failure due to oxalosis despite treatment with pyridoxine since the age of 4 months. rhe 3rd child of this family was found to have hyperoxalria type I at the age of 3 weeks:plasma oxalate 6 0 umol/l (n:3-23) ,plasma glycollate 1157 umol/l (n:7-33) ,urikary 2xalate 1650 mmol/rnol creat. We report on a male Turkish neonate of 38w gestation with a birthweight of 2500 g. After normal adaptation, clinical and radiological signs of cardiomegaly were noticed on the 2nd day in the absence of muscular hypotonia. Echocardiography revealed hypertrophy involving minly the right ventricle (RV) and the septum but less the left ventricle: RV anterior wall diameter and septum thicknesss were 6.0 and 6.5 mm, resp. (normal R 2 SD 2.0 2 0.3 and 2.5 t 0.4, resp.) on days two and seven. Serum C levels were low on day two (total C 15, free C 6.5 umol/l, resp.; normal: 36 -62 and 21 -56, resp). Oral substitution with L-carnitin (100 mglkg bwlday) was given from day 7 to 28. Cardiomegaly, echocardiographic parameters and serum C normalized within 10 days and remained normal for the following 3 mos. Since placental C transfer is a passive process we considered C deficiency in his mother. Four weeks after delivery, her serum C was 20 and 12 pmol/l, the muscle tone was normal, ketogenesis unimpaired and urinary excretion of dicarbonic acids absent. Excessive renal loss was ruled out (tubular reabsorption >99 5 ) . However, a low alimentary intake due to a self-imposed exclusion of ...

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