LDL-cholesterol determination in blood serum following precipitation of LDL with polyvinylsulfate

Assmann, Gerd; Jabs, Hans-Ulrich; Kohnert, Ulrich; Nolte, W.; Schriewer, H

doi:10.1016/0009-8981(84)90153-0

Cited by 173 publications

(97 citation statements)

References 14 publications

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“…Plasma calcium [20], phosphorus [21], magnesium [22], zinc [23], glucose and cholesterol [24], LDL [25], HDL [26], triglycerides [27], MDA [28], and TAC [29] were estimated using colorimetric method commercial kits from Biodiagnostic Egypt.…”

Section: Discussionmentioning

confidence: 99%

Rankl Expression and Metabolic Changes in Ovariectomized Rats and the Possible Protection by Vegetable Formula.

Hanafi

2018

Asian J Pharm Clin Res

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Objective: This study was to evaluate complications of osteoporosis in ovariectomized rats and the possibility to ameliorate these changes by consumption of vegetable formula. Furthermore, transcription of mRNA of RANKL gene was matched with bone mass density (BMD) and bone formation marker (human procollagen 1 N terminal peptide [PINP]).Methods: Thirty rats were divided into three groups. The first is non ovariectomized control group (NOVXC), the second is ovariectomized control group (OVXC), and the third is ovariectomized rats supplemented with the vegetable formula (OVXT). Animals were fed for 8 successive weeks. Animals were treated and sacrificed under the recommended ethics of laboratory animal's treatment. The vegetable mixture was formulated with the purpose to correct the bone compromise and supply all the presumed deficient elements and hormone.Results: Chemical analysis showed that the formulated vegetable mixture had a high amount of flavonoids as catechin (100 mg/100 g of dry weight) and polyphenols as tannic acid (1000 mg/100 g dry weight. Furthermore, it had high reducing power (1,1-diphenyl-2-picrylhydrazyl radical showed inhibition percentage of 91.81%.). Several phytochemicals necessary for bone health were demonstrated in the vegetable mixture using highperformance liquid chromatography. RANKL/GAPDH mRNA transcription ratio showed marked an increase in OVXC versus the control NOVXC rats Conclusion:It may be concluded that the plant formula was effective to minimize health hazards in ovariectomized rats and maybe for postmenopause women. Perhaps longer time may be needed for more significant and clear effect.

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Section: Discussionmentioning

confidence: 99%

Rankl Expression and Metabolic Changes in Ovariectomized Rats and the Possible Protection by Vegetable Formula.

Hanafi

2018

Asian J Pharm Clin Res

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“…High density lipoprotein cholesterol was measured after isolation by selective precipitation with dextran sulphate 500,000 Mg Cl 2 9 . Low density lipoprotein cholesterol was calculated according to Assmann's formula 1 .…”

Section: Methodsmentioning

confidence: 99%

Blood lipid associations in 18 year-old men

1992

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POLETTO, L. et al. Blood lipid associations in 18 year-old men. Rev. Saúde públ, S. Paulo, 26: 316-20, 1992. The association of cigarette smoking, physical activity at work, and social class with total cholesterol and with high and low density lipoprotein cholesterol were examined in a random sample of 238 males, of 18 years of age, of Rosario, Argerntina. The mean (mg/dl) total serum cholesterol of the whole sample was 174.7, the high density lipoprotein cholesterol 52.8, and the low density lipoprotein cholesterol 121.5. Black tobacco consumers, evenly distributed by social class, had higher levels of total and low density lipoprotein cholesterol. Total cholesterol was higher in the high social class, differently from what smokers' distribution by social class, would lead one to expect. While a highly negative association was found between social class and physical activity at work, there were no significant diferences in lipoprotein levels between manual and non-manual workers. It is possible that the nutritional differences by social class still prevail over the smoking habit in their influence on the lipoprotein levels in these subjects.

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“…After measuring the absorbance at 520 nm, the cholesterol in the supernatant was determined by comparison with the calibrator. The LDL-C content was then quantified by subtracting the cholesterol in the supernatant from total cholesterol (Assmann et al, 1984).…”

Section: Analysis Of Triglyceride (Tg) High Density Lipoprotein-cholmentioning

confidence: 99%

The effects of bamboo leaf extract on growth performance, antioxidant traits, immune function and lipid metabolism of weaning piglets

Zhang¹,

Wang²,

Zhang³

et al. 2013

J. Anim. Feed Sci.

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LDL-cholesterol determination in blood serum following precipitation of LDL with polyvinylsulfate

Cited by 173 publications

References 14 publications

Rankl Expression and Metabolic Changes in Ovariectomized Rats and the Possible Protection by Vegetable Formula.

Rankl Expression and Metabolic Changes in Ovariectomized Rats and the Possible Protection by Vegetable Formula.

Blood lipid associations in 18 year-old men

The effects of bamboo leaf extract on growth performance, antioxidant traits, immune function and lipid metabolism of weaning piglets

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