Sisomicin, the principal antibiotic produced in the fermentation of Micromonospora inyoensis, has been shown to be 0-2,6-diamino-2,3,4,6-tetradeoxy-a-D-gZycero-hex-4-enopyranosyl(l-4)-0-[3-deoxy-4-C-methyl-3-(methylamino)-/?-L-arabinopyranosyl(l-6)]-2-deoxy-D-streptamine (1). Sisomicin contains a novel unsaturated sugar unit, not previously encountered in any aminocyclitol antibiotic.Submerged fermentations of Micromonospora inyoensis (NRRL 3292) produce sisomicin,1 a novel unsaturated aminocyclitol antibiotic2•3 having broad spectrum antibacterial activity.4 Sisomicin is the major component of the crude antibiotic complex, which was isolated from the fermentation broth by ion-exchange chromatography.3 Column chromatography of the crude antibiotic on silica Preparative Routes to 4-Amino-4-deoxy-D-galactose1
A mutant strain of Micromonospora purpurea, designated var. JI-33, produced an antibiotic complex consisting primarily of gentamicin Cla,. A further product of this fermentation was identical to a very minor component isolated from the fermentation of the parent organism and named gentamicin C22. Physical measurements indicated its structure to be 6'-N-methylgentamicin C1a, and this was confirmed by synthesis from gentamicin Cla. The in vitro antibacterial activity of gentamicin C2b was very similar to that of the gentamicin C complex. Antibiotic XK-62-2, produced by Micromonospora sagamiensis, appears to be identical to gentamicin C22. Fermentation of Micromonospora purpurea NRRL 2953 affords, as the major products, the important antibiotics gentamicins C1, C2 and C1a , (gentamicin C complex)2) as well as a host of minor components.') During the screening of this organism, in search of improved gentamicin producers, a novel variant, designated M. purpurea var. JI-33, was isolated. The strain, obtained by multiple nitrosoguanidine treatments and repeated strain selection, was found to possess similar morphology to that already described for gentamicin-producing strains of M.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.