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In this study, we report the development of a screening method for highly accurate and sensitive analysis of 44 cephalosporins in 4 representative meats (pork, beef, chicken, and mutton).

Isoflavones are natural substances that exhibit hormone-like pharmacological activities. The separation of isoflavones remains an analytical challenge because of their similar structures. We show that ultra-high performance supercritical fluid chromatography can be an appropriate tool to achieve the fast separation of 12 common dietary isoflavones. Among the five tested columns the Torus DEA column was found to be the most effective column for the separation of these isoflavones. The impact of individual parameters on the retention time and separation factor was evaluated. These parameters were optimized to develop a simple, rapid, and green method for the separation of the 12 target analytes. It only took 12.91 min using gradient elution with methanol as an organic modifier and formic acid as an additive. These isoflavones were determined with limit of quantitation ranging from 0.10 to 0.50 μg/mL, which was sufficient for reliable determination of various matrixes.

A simple, accurate, and highly sensitive method was developed for the determination of 13 carbohydrates in polysaccharide of Spirulina platensis based on high-performance anion-exchange chromatography coupled with pulsed amperometric detection and mass spectrometry. Samples were extracted with deionized water using ultrasonic-assisted extraction, and the ultrasound-assisted extraction conditions were optimized by Box-Behnken design. Then the extracted polysaccharide was hydrolyzed by adding 1 mol/L trifluoroacetic acid before determination by high-performance anion-exchange chromatography coupled with pulsed amperometric detection and confirmed by high-performance anion-exchange chromatography coupled with mass spectrometry. The high-performance anion-exchange chromatography coupled with pulsed amperometric detection method was performed on a CarboPac PA20 column by gradient elution using deionized water, 0.1 mol/L sodium hydroxide solution, and 0.4 mol/L sodium acetate solution. Excellent linearity was observed in the range of 0.05-10 mg/L. The average recoveries ranged from 80.7 to 121.7%. The limits of detection and limits of quantification for 13 carbohydrates were 0.02-0.10 and 0.2-1.2  μg/kg, respectively. The developed method has been successfully applied to ambient samples, and the results indicated that high-performance anion-exchange chromatography coupled with pulsed amperometric detection and mass spectrometry could provide a rapid and accurate method for the simultaneous determination of carbohydrates.

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A multiflavor detection method, using gas chromatography–triple quadrupole tandem mass spectrometry (GC-MS/MS), has been developed for the simultaneous identification and quantification of eight prohibited flavor compounds in daily foods. Under the optimized extraction conditions, samples were purified directly through membrane filtration. Variables affecting the GC-MS/MS were optimized to obtain better separation. The excellent selectivity and sensitivity achieved in multiple reactions monitoring mode allowed satisfactory confirmation and quantitation. In this study, the linear ranges of the target compounds were 0.05 to 500 ng/L with good correlation coefficients (R2 > 0.999). The limits of detection of target compounds ranged from 0.005 to 0.2 μg/kg. The average recoveries were in the range of 80.2 to 110.6% (beef jerky), 82.3 to 94.1% (cod liver oil), and 83.6 to 104.1% (candy).

J. Sep. Sci. 2017, 40, 2827–2837 DOI: https://doi.org/10.1002/jssc.201601454 The cover picture shows that ultra‐high performance supercritical fluid chromatography could be an appropriate tool to achieve baseline separation of twelve common dietary isoflavones, which possess the same skeleton structure. The elution order of isoflavones was mainly attributed to polar interactions, which led to increased retention of compounds with increased molecular polarity corresponding to the behavior in normal‐phase liquid chromatography. For example, the dipole moment is in positive correlation with molecule polarity. The value of puerarin is bigger than that of daidzin so that former was eluted after the latter.