Examination of the molecular mechanism of taurine regulation of lipid
metabolism in fish is limited. In this study, an oleic acid (OA)-induced
hepatocyte steatosis model of orange-spotted grouper (Epinephelus coioides)
was established for the first time. The model was used to test the effect of
taurine on steatosis hepatocytes in Control, High-fat (0.4 mM OA) and
Taurine (0.4 mM OA + 2 mM taurine) experimental groups of fish. Hepatocyte
samples were subjected to transcriptome analysis. A total of 99634 unigenes
was assembled, 69982 unigenes were annotated and 1831 differentially
expressed genes (DEGs) in Control vs High-fat group, and 526 DEGs in the
High-fat vs Taurine group were identified, of which 824 DEGs (Control vs
High-fat) and 237 DEGs (High-fat vs Taurine) were observed to be
upregulated, and 1007 DEGs (Control vs High-fat) and 289 DEGs (High-fat vs
Taurine) were downregulated after taurine intervention. These genes are
involved in peroxisome proliferator-activated receptor (PPAR) and 5'
AMP-activated protein kinase (AMPK) signaling pathways, fatty acid
elongation, primary bile acid biosynthesis, glycerophospholipid and
glycerolipid metabolism. The findings provide new clues in understanding the
regulatory role of taurine in lipid and fatty acid metabolism of fish. It is
hoped that the obtained results will help in the design of feed formulations
to improve grouper growth from the perspective of aquaculture nutrition.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.