Growth and metabolic responses of grouper juveniles (Epinephelus coioides) fed diets containing varying levels of leucine

Dietary lactoferrin supplementation improves growth performance and intestinal health of juvenile orange-spotted groupers (Epinephelus coioides)

“…Intact intestinal histomorphology is a prerequisite for maintaining good digestion and absorption state and intestinal immune disease resistance [69]. The histomorphological indicators such as lMF, tML, and nMF were used to evaluate intestinal digestion and absorption capacity in previous studies [1,19]. In the present study, a signi cant reduction in tML in DI was observed in SBM diets vs FM diets, as evidenced by previous study [1].…”

“…PI, MI, and DI, respectively), according to the method described by Anguiano et al [27]. All the segments were washed with normal saline, xed in Bouin's solution for 24 h, rinsed with 70% ethanol solution, and nally immersed in 70% ethanol until histological processing was performed [19]. The xed gut segments were embedded in para n and 5-µm sections were cut by using a rotary microtome (KD-2258S, China).…”

“…Orange-spotted grouper (Epinephelus coioides), a marine economic carnivorous sh, which is widely cultured in Southeast Asian countries including China [19,20]. This sh has become the third largest mariculture sh in China, with an annual output of 192,045 tons in 2020 [21].…”

Dietary sodium butyrate administration alleviates high soybean meal-induced growth retardation and enteritis of orange-spotted groupers (Epinephelus coioides)

“…To perform histological analysis, one fish was caught from each tank and dissected to obtain the whole gut, then divided into proximal, middle, and distal intestines (i. e. PI, MI, and DI, respectively), according to the method (Anguiano et al, 2013). All the segments were washed with normal saline, fixed in Bouin's solution for 24 h, rinsed with 70% (v/v) ethanol solution, and finally immersed in 70% (v/v) ethanol until histological processing was performed (Niu et al, 2021). The fixed gut segments were embedded in paraffin and 5-µm sections were cut by using a rotary microtome (KD-2258S, China).…”

Growth performance, biochemical composition and expression of lipid metabolism related genes in groupers ( Epinephelus coioides ) are altered by dietary taurine

“…However, the levels of body lipid, plasma cholesterol, and blood glucose were reduced by taurine intervention in fish (Martins et al, 2021; Michelato et al, 2018; Zhang et al, 2019), suggesting that taurine plays a critical role in lipid metabolism in fish. Grouper ( Epinephelus coioides ) is a carnivorous fish species that has gained increased importance in the aquaculture industry (Niu et al, 2021; Shapawi et al, 2019). In China, groupers are ranked as the second mariculture fish species, with a production of 183,127 tons in 2019 (China Fishery Statistics Yearbook, 2020).…”