A cross-sectional study was conducted on organized Dairy Farm at Asella, Oromia Regional State of Ethiopia to determine seroprevalence and risk analysis of bovine brucellosis in May, 2016. A total of 304 samples were collected; all were tested and confirmed serologically using Rose Bengal plate test (RBPT) and complement fixation test (CFT). Out of 304 samples tested, overall seroprevalence of RBPT and CFT results was12.48% (38) and 9.87% (30) respectively, which was higher in animals above two years age than younger one. History of abortion and retained fetal membrane were found to be significantly (p<0.05) associated with occurrence of bovine brucellosis. A statistically not significant difference (p >0.05) was observed between cross and local dairy cattle. The result showed the high prevalence of bovine brucellosis in the farm. Hence, culling of the positive dairy cattle and practicing good management should result in a control and prevent of the brucellosis.
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