Carbon dioxide capture, conversion, and utilization with
carbonic
anhydrase (CA) as a green and sustainable method still faces the challenges
of complex and costly purification, relatively low yield, and poor
catalytic performances and reusability. Herein, we proposed an all-in-one
strategy to solve these problems by self-assembling nanosized CA oligomeric
particles (nCAOPs) into micrometer-sized CA supraparticles (mCASPs)
with well-designed tags. The preparation of the purified mCASPs was
simple and effective by an easy scalable low-speed centrifugation
method with 78% activity recovery. The obtained mCASPs with a yield
of 870 mg/L was the reported highest yield of CAs. Interestingly,
mCASPs could serve as carrier-free immobilized CAs and retain over
90% original activity after 15 reuse cycles. More encouragingly, mCASPs
could redissolve and form nCAOPs, which had excellent catalytic performances.
The hydrated activity of nCAOPs was 1.05 times that of free CAs. Also,
the k
cat/K
m value was 1.74 times that of free CAs, and the half-life at 40 °C
was 3.83 times that of free CAs. Due to the simplicity of purification
and immobilization, high yield, and excellent enzymatic properties,
mCASPs and nCAOPs are considered novel bioactive materials, which
offer the feasibility of CAs for sustainable CO2 capture
to achieve the target of carbon neutrality.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.