Biological information is obtained from the interaction between the series detection electrode and the organism or the physical field of biological cultures in the non-mass responsive piezoelectric biosensor. Therefore, electric parameter of the electrode will affect the biosensor signal. The electric field distribution of the microelectrode used in this study was simulated using the COMSOL Multiphysics analytical tool. This process showed that the electric field spatial distribution is affected by the width of the electrode finger or the space between the electrodes. In addition, the characteristic response of the piezoelectric sensor constructed serially with an annular microelectrode was tested and applied for the continuous detection of Escherichia coli culture or HeLa cell culture. Results indicated that the piezoelectric biosensor with an annular microelectrode meets the requirements for the real-time detection of E. coli or HeLa cells in culture. Moreover, this kind of piezoelectric biosensor is more sensitive than the sensor with an interdigital microelectrode. Thus, the piezoelectric biosensor acts as an effective analysis tool for acquiring online cell or microbial culture information.
Pulse shaping techniques are discussed in this paper for the practicability of a developed high-g shock tester. The tester is based on collision principle where there is a one-level velocity amplifier. A theoretical and experimental study of pulse shaping techniques is presented. A model was built and theoretical formulae were deduced for the shock peak acceleration and its duration. Then theoretical analysis and some experiments were conducted. The test results verify the validity of theoretical model and show that the shock tester can generate the expected high-g shock pulses by integrated usage of different impact velocities and pulse shapers made from different materials. This is important in practical applications where the items under test can be shown to excite specific resonances at predetermined acceleration levels using the shock tester.
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