On-Line Monitoring the Growth of E. coli or HeLa Cells Using an Annular Microelectrode Piezoelectric Biosensor

Abstract

Biological information is obtained from the interaction between the series detection electrode and the organism or the physical field of biological cultures in the non-mass responsive piezoelectric biosensor. Therefore, electric parameter of the electrode will affect the biosensor signal. The electric field distribution of the microelectrode used in this study was simulated using the COMSOL Multiphysics analytical tool. This process showed that the electric field spatial distribution is affected by the width of the electrode finger or the space between the electrodes. In addition, the characteristic response of the piezoelectric sensor constructed serially with an annular microelectrode was tested and applied for the continuous detection of Escherichia coli culture or HeLa cell culture. Results indicated that the piezoelectric biosensor with an annular microelectrode meets the requirements for the real-time detection of E. coli or HeLa cells in culture. Moreover, this kind of piezoelectric biosensor is more sensitive than the sensor with an interdigital microelectrode. Thus, the piezoelectric biosensor acts as an effective analysis tool for acquiring online cell or microbial culture information.

Rapid Detection of Broad-Spectrum Pathogenic Bacteria Based on Highly Sensitive Proton Response of the Nucleic Acid Amplification SPQC Platform

“…Previous investigations have established that P 1 and P 2 are constants spanning several orders of magnitude in actual analyses . Consequently, after connecting 2D PANI electrodes in series into the device (Figure S4b), this device can acutely capture small electrical parameter changes, resulting in pronounced piezoelectric frequency alterations.…”

“…Under the working conditions of PQC, differentiating the above formula yields a new frequency curve model, which reflects that the sensitivity of the PR-SPQC system is jointly determined by C t and Previous investigations have established that P 1 and P 2 are constants spanning several orders of magnitude in actual analyses. 42 Consequently, after connecting 2D PANI electrodes in series into the device (Figure S4b), this device can acutely capture small electrical parameter changes, resulting in pronounced piezoelectric frequency alterations. Due to its affordability and exceptional signal amplification characteristics, these features make SPQC exceptionally well suited for the construction of the PR-SPQC biosensing platform.…”

Influence of Electrode Structure on Performance of Laser Direct Writing Cu-PI Flexible Humidity Sensor

“…generated by different electrode structures. Vakilian et al [ 18 ] obtained the optimum size of interdigital electrode for biosensors by COMSOL software to analyze the capacitance value of the sensor influenced by different electrode structural parameters. The simulation results showed that the total capacitance of the electrode with the interdigital length of 100 μm increased by 50% when the interdigital width increased from 10 μm to 25 μm, the capacitance decreased when the interdigital distance fell from 25 μm to 10 μm, and the interdigital thickness had no significant effect on the interfinger capacitance, which proved that the optimum purpose could be attained by adjusting the interdigital width and distance to change the electrode characteristics.…”

The Detection Method ofEscherichia coliin Water Resources: A Review

“…Recently, F. Tong et al developed the piezoelectric biosensor with an annular microelectrode as shown in Figure 8. By using micro-scale structure of microelectrode, the optimization in term of current density, rapid response time and mass transfer between the electrodes [62]. The benefit of using piezoresistivity method is that the detection mechanism can be integrated easily into lab-on-a-chip type devices.…”