The toxins of a xanthid crab Atergatis floridus from Chiba were purified successively on acti vated charcoal, Bio-Gel P-2, and Bio-Rex 70, and then analyzed by thin layer chromatography and electrophoresis. No significant difference was observed between August and November specimens . In Bio-Rex 70 column chromatography, 99 % of the toxins were eluted with 0-0.03 and 1% with 1.5N acetic acid; these results indicated that the crabs in Chiba contain gonyautoxins as major toxic com ponents, differing from those in Okinawa in which saxitoxin and neosaxitoxin are predominant. Three toxins, none of which were in accord with the standards used , were detected in the gonyautoxin fraction. One of them was the major toxic component, since its relative ratio to the total toxicity was as high as 85-91%.
The antihypertensive effect of yeast extract mannan was tested on male spontaneously hypertensive rats (SHR).Mannan purification was performed by acid-methanol precipitation and gel filtration on Bio gel A-0. 5 m. The structure of the isolated mannan was confirmed by infrared and 13C-NMR spectroscopies, and sugar analysis. The antihypertensive effect of yeast mannan was clear at 50100 mg/kg at 2'.-6 hr after intraperitoneal administration, and at 100-200mg/ kg at 4-6hr after oral administration. When administered intraperitoneally, yeast mannan was equal to a-methyldopa (positive control) in antihypertensive activity. A commercially available mannan from Saccharomyces cerevisiae also lowered SHR blood pressure.
Toxins in ovaries and livers of the puffer fish, Fugu flavidus, were purified successively on activated charcoal, Bio-Gel P-2 and Bio-Rex 70. Besides tetrodotoxin (TTX), a minor toxin (toxin D) was newly detected in both tissues; toxin Daccounted for 3%and 4%of the totl lethal potency in ovaries and livers, respectively. Although there were differences in the specific lethal activity, dose-survival time relation and signs in mice between toxin D and TTX, positive reactions to Weber reagent and 10% KOH,and a negative one to 1% H2O2were observed for both toxins. Moreover, toxin D, like TTX, gave rise to the C9-base upon alkali-degradation.These results suggested that toxin D is a TTX-like compoundhaving a quinazoline skeleton.
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