The inclusion behavior of sulfobutylether-β-cyclodextrin (SBE-β-CD) with perphenazine (PPH) was first studied by flow injection (FI)-chemiluminescence (CL) analysis with proposed lg[(I 0 -I s )/I s ] = lgK P-CD + nlg[C PPH ] model and molecular docking. Results showed that a 1:1 complex of SBE-β-CD/PPH could online form, with the formation constant K P-CD of 2.57 × 10 7 L mol −1 at 298 K. The thermodynamic parameters showed that the inclusion behavior of SBE-β-CD/PPH was a spontaneous process by hydrophobic interaction. The molecular docking results revealed PPH entered into the larger cavity of SBE-β-CD with two hydrogen bonds. Based on the linear relationship of the decrement of luminol/SBE-β-CD/PPH CL intensity against the logarithm of PPH concentration ranging from 0.03 to 30.0 ng mL, the present FI-CL analysis using luminol/ SBE-β-CD/PPH system was successfully applied to PPH determination in biological fluids and tablets with recoveries from 94.5 to 105.6% and RSDs less than 2.6% (n = 5).
An ultrasensitive method for determining picomolar midecamycin (MID) by flow injection (FI) chemiluminescence (CL) was first described based on the inhibitory effect of MID on luminol–BSA reaction. It was found that the CL intensity decrements were linear with the logarithm of MID concentrations in the range of 1.0–5000 pmol · l–1with a detection limit as low as 0.3 pmol · l–1(3σ). The relative standard deviation of seven repetitive measurements for 10 pmol · l–1MID was 3.0%. At a flow rate of 2.0 ml · min–1, the whole analysis procedure including sampling and washing could be finished in 30 s, offering the sample efficiency of 120 h–1. This proposed method was successfully applied to determine MID in human serum samples with the recoveries from 96.0 to 110.0%. The CL mechanism of luminol–BSA–MID reaction was also given.
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