Ultrasensitive determination for picomolar-level midecamycin in human serum by flow injection chemiluminescence using luminol–BSA system

Abstract

An ultrasensitive method for determining picomolar midecamycin (MID) by flow injection (FI) chemiluminescence (CL) was first described based on the inhibitory effect of MID on luminol–BSA reaction. It was found that the CL intensity decrements were linear with the logarithm of MID concentrations in the range of 1.0–5000 pmol · l–1with a detection limit as low as 0.3 pmol · l–1(3σ). The relative standard deviation of seven repetitive measurements for 10 pmol · l–1MID was 3.0%. At a flow rate of 2.0 ml · min–1, the whole analysis procedure including sampling and washing could be finished in 30 s, offering the sample efficiency of 120 h–1. This proposed method was successfully applied to determine MID in human serum samples with the recoveries from 96.0 to 110.0%. The CL mechanism of luminol–BSA–MID reaction was also given.

A voltammetric sensor based on electrochemically reduced graphene modified electrode for sensitive determination of midecamycin

“…1,2 Owing to the extensive use in infectious disease therapy, the determination of MD is essential to optimize the therapy and to avoid toxic concentrations. Various analytical methods have been reported for the determination of MD, which include high performance liquid chromatography (HPLC), 3 liquid chromatography-mass spectrometry (LC-MS), 4 supercritical fluid chromatography-mass spectrometry (SFC-MS), 5 spectrophotometry, 6 flow injection chemiluminescence (FI-CL), 7 capillary zone electrophoresis (CZE), 8 thin-layer chromatography, 9 etc. Some of these reported methods suffer from disadvantages such as complicated procedures, long analysis time, expensive instrumentation, and relatively low selectivity or sensitivity.…”