Objectives: To explore an association between CAPN10, SNP-44 (rs2975760) with IR condition in women with PCOS. Methods: A study included 120 participants of which 68 women have PCOS subdivide according to their body mass index (BMI) into 45 obese (BMI≥30) and 23 non-obese (BMI<30). The remaining 52 represent the control group who were apparently healthy women with normal weight and normal menstrual cycle. Patients with PCOS were selected from the Infertility Department, Gynecology and Obstetrics Teaching Hospital, Kerbala Health Directorate / Kerbala-Iraq between Nov., 2021 and June, 2022. Diagnosis of PCOS is based on 2 of 3 findings: oligo/anovulation, hyperandrogenism, polycystic ovaries in ultrasound (Rotterdam criteria). Patients were interviewed and examined for weight, height, waist circumference, and hip circumference. Venous blood samples were collected at 9 AM after an overnight fast. IR was assessed by calculating homeostatic model assessment of insulin resistance (HOMA-IR) using the formula (fasting glucose mg/dl x fasting insulin µU/ml)/405, taking normal value <2.7. Genotypes of CAPN10, SNP-44 has been identified using Allele-specific polymerase chain reaction (AS-PCR) technique. Results: The prevalence of IR based on HOMA-IR was (80%) in obese PCOS and (48%) in non-obese PCOS women. CAPN10, SNP-44 has been reconstructed and analyzed in patients and controls. Genotypes of 45 obese PCOS subjects (TT, N=26; TC, N=12; and CC, N=7), 23 non-obese PCOS subjects (TT, N=15; TC, N=6; and CC, N=2) and control subjects (TT, N=39; TC, N=11; and CC, N=2) were identified. The genotype distribution was statistically different between obese PCOS women and controls (OR=5.25, P=0.048). The association of SNP-44 allele with IR status was detected. HOMA-IR was greater in CC (10.54 ± 1.29, 9.88 ± 1.41) than in TT (3.30 ± 1.52, P<0.001; 2.82 ± 1.45, P<0.001) and TC (3.76 ± 1.58, P<0.001; 4.10 ±1.57, P<0.05) in obese PCOS and non-obese PCOS subjects respectively. Conclusion: In obese PCOS, the C allele was associated with higher insulin secretion and HOMA-IR compared with the T allele. The increased HOMA-IR is an indicator of IR. In this scenario, the C allele might be involved in the pathophysiology of insulin resistance in PCOS.
Objectives: To assess the insulin resistance by determination of C-peptide and triglyceride levels in women with polycystic ovarian syndrome (PCOS) and then investigation of insulin resistance by various methods and comparing them with the homeostatic model assessment method used. Methods: A study included 90 participants of which 55 women have PCOS subdivide according to their BMI to 32 obese (BMI>=30) and 23 non-obese (BMI<30). The remaining 35 represent the control group who were apparently healthy women with normal weight and normal menstrual cycle. Patients with PCOS were selected from the Infertility Department, Gynecology and Obstetrics Teaching Hospital, Kerbala province, Iraq between Nov., 2021 and June, 2022. Diagnosis of PCOS is based on 2 of 3 findings: oligo/anovulation, hyperandrogenism, polycystic ovaries in ultrasound (Rotterdam criteria).The patients were interviewed and examined for weight, height, waist circumference, and hip circumference. Venous blood samples were collected at 9 AM after an overnight fast. Measurement of serum insulin, glucose, triglyceride and c-peptide were performed using Cobas instrument and by ELISA technique. Results: Based on HOMA-IR, the prevalence of insulin resistance in obese PCOS women was 77% while in non-obese PCOS women was 70%. HOMA-IR, QUICKI, McAuley and CPI showed significant difference between obese PCOS (4.39 ± 1.83), (0.309 ± 0.024), (3.85 ± 0.91) and (4.39 ± 1.63) respectively; and control group (2.68 ± 0.61), (0.331 ± 0.011), (4.53 ± 0.57) and (8.72 ± 1.33) respectively. CPI also showed significant difference between obese PCOS (4.39 ± 1.63) and non-obese PCOS (6.85 ± 1.74). In obese PCOS women, both QUICKI (r = -1.00, P < 0.001) and McAuley (r = -0.81, P < 0.001) were strongly correlated with HOMA-IR, whereas CPI was not. For non-obese PCOS, there was a strong correlation for both QUICKI (r = -0.97, P < 0.001), (r = -0.62, P < 0.05) and HOMA-IR, CPI was also strongly correlated with HOMA-IR (r = -0.78, P < 0.001). Conclusion: Significant number of women with PCOS can be classified as being either insulin sensitive or insulin resistant (IR) depending on the method applied, as correlation between various IR indices is highly variable. Clinical application of surrogate indices for assessment of IR in PCOS must be therefore viewed with an extreme caution.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.