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In order to provide a better characterization and understanding of the brine shrimp Artemia salina life-cycle, different ecological and biological parameters were taken out monthly during two periods from Sabkhet El Adhibet (south-east Tunisia). Variation of water temperature, salinity, pH, dissolved oxygen, nutrients (orthophosphate, nitrites, nitrates and ammonium) and phytoplankton density were monitored. The Artemia population was also surveyed. Artemia were present in the site with salinity between 32.2 and 281.7 g l 21 and water temperature between 12.1 and 25.48C. The pH ranged from 7.6 to 9 and dissolved oxygen concentration from 3.4 to 17.5 mg l 21 . Minimum and maximum values of phytoplankton density were 0.19 and 14.59 10 6 cell l 21 . In addition, the nutrient analysis registered showed that nitrate and ammonium represent the major nutrient. The Artemia population density fluctuated between 0.22 and 38.57 individuals per litre. The male:female ratio was dominated by the males. Artemia from Sabkhet El Adhibet showed variability in fecundity (total offspring and brood size) as well as in the ratio encystment/oviviparity. The individual fecundity fluctuates between 29.4 and 70.2 cysts and 17.8 and 69.8 nauplii. Finally, the influence of physical and chemical parameters as well as phytoplankton density over the Artemia population was noticed.