Subepithelial connective tissue grafts (SCTGs) with a coronally advanced flap (CAF) are accepted as the gold standard for covering denuded root surfaces. In recent years, enamel matrix derivatives (EMDs) have been used for their regenerative potential in periodontics. The aim of this split-mouth and randomized controlled study was to assess the clinical and aesthetical impacts of EMD application in combination with SCTG+CAF in patients with Miller's Class I and II gingival recessions in contralateral canines of the maxilla. Participants who underwent SCTG+CAF+EMD application were identified as the test group (n = 19) and those who underwent SCTG+CAF as control group (n = 19). The outcome parameters were recession depth/width, root coverage percentage, and root coverage aesthetic score (RES). RES was evaluated by two calibrated blind periodontists one year after the treatment. Statistically significant root coverage percentage was observed at one year post-treatment for both groups (p < 0.05). However, significant differences between the groups were not observed in terms of total RES and complete root coverage rate (p > 0.05). The test group had significantly better results than the control according to the soft tissue texture and mucogingival junction alignment results (p < 0.05). These results indicate that EMDs contribute to the healing of soft tissue without scarring. As a result of better wound healing, the EMD-added group exhibited better results in terms of the harmony of the mucogingival junction between adjacent teeth. This paper is the first split-mouth study in which SCTG+CAF and SCTG+CAF+EMD were compared using RES in bilateral canines.

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Objectives This study aimed to investigate the effects of chronic restraint stress on the osseointegration of titanium implants. Materials and Methods Twenty adult male Wistar albino rats were used in the study. After surgical insertion of titanium implants into the metaphyseal part of the tibial bone, rats were randomly divided into two groups: a control group (CNT group) and an experimental restraint stress group (RS group). In the CNT group, titanium implants were inserted surgically, and rats received no further treatment during the 47-day experimental period. In the RS group, restraint stress was applied for 3 hours per day for 45 days, beginning 2 days after implant surgery. Weight of the rats was measured prior to surgery and at the end of the study to analyze the effects of stress. At the end of the experimental period, rats were euthanized, and implants and surrounding bone tissues were used for undecalcified histological analysis. Serum cortisol levels were assessed in cardiac blood samples from the rats following centrifugation. Results Average weight of rats in the RS group was lower than that of rats in the CNT group after the experimental protocol had been completed ( P <0.05). Further, serum cortisol levels were higher in the RS group than in the CNT group ( P <0.05). There were no significant differences in bone-implant connection levels between the two groups ( P >0.05). Conclusion The data analyzed in this study suggest that chronic restraint stress did not adversely affect rats during a 45-day osseointegration period.

Objective: In this study, randomized clinical trial (RCT), the aim was to compare clinical periodontal data of subepithelial connective tissue graft (SCTG) group and SCGT+enamel matrix derivatives (EMD) group in terms of gained gingiva volume (GGV) and gained gingiva surface area (GGSA) by scanners of computeraided design/computer-aided manufacturing (CAD/CAM) devices. Material and Methods:In this study, a total of 35 Miller class I-II gingival recession defects were involved (n=17 in SCTG group, n=18 in SCTG+EMD group). In addition to, periodontal clinical parameters, and three dimensional (3D) images were recorded by using CAD/CAM system before the treatment, at the 1 st month and the 6 th months after the treatment. The images were superimposed by using softwares and GGV, and GGSA were calculated. Results:There was no significant difference in terms of GGV and GGSA values at the 1 st month and 6 th months between SCTG+EMD and SCTG groups. Similarly, there was no significant difference in periodontal clinical parameters between groups. Conclusion:Images obtained from CAD/CAM system can be easily used in calculating GGV and GGSA data after the gingival recession treatment and in determining of the success of administered treatment. It is expected that the evaluation of the success of many therapeutic approaches for periodontal treatment will be carried out by using CAD/CAM system in the future.

Objectives The present study aims to evaluate patients' knowledge about toothbrush disinfection (TBD), plaque control, knowledge, and awareness of periodontal diseases (P‐knowledge and P‐awareness). Methods The study enrolled 121 participants (70 women and 51 men). Participants were asked to fill out a survey containing questions about demographic characteristics, toothbrushing habits, toothbrush storage conditions, toothpaste use, TBD, P‐knowledge, and P‐awareness. The periodontal clinical parameters (GI, PI, PPD, CAL, BOP, and GR) and DMFT index were recorded. The patients were informed about TBD, and 1 month later, the participants were contacted again and asked whether they disinfected their toothbrushes. Results The participants (51.2%) do not have any knowledge about TBD. Most of those who reported disinfecting their toothbrushes think that washing toothbrushes with tap water is disinfection (23.1%). One month after the TBD training, only 36.3% of the participants reported that they disinfected, albeit not regularly, their toothbrushes with white vinegar. While there was no statistically significant relationship between genders in terms of visiting dentists, a significant relationship was found between genders in terms of toothbrushing frequency (p < 0.05). P‐awareness was found to be “medium” while P‐knowledge was at a “low” level. While there was no statistically significant difference between income levels in terms of P‐knowledge levels, a significant difference was found between genders and educational backgrounds in terms of P‐knowledge levels (p < 0.05). Conclusions The results of the study suggest that the participants do not have enough knowledge about toothbrush storage conditions, toothpaste sharing, and TBD. Collaborating with dental hygienists to raise awareness about oral health and TBD may have a positive impact.

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