Diagnostic molecular markers for the females of Phlebotomus (Paraphlebotomus) caucasicus and P. mongolensis were sought by characterizing from individual Iranian specimens a gene fragment, namely mitochondrial cytochrome b, that had previously proven useful for the taxonomy of phlebotomine sandflies. Males of both species were used as reference material because their external genitalia provide the only diagnostic morphological characters. A phylogenetic analysis of the new sequences, and those previously reported for P. grimmi, found no support for recognizing more than one species (P. caucasicus s.l.) in Iran. Most of the genetic variation was geographical. An absence of lineage sorting was demonstrated, and it is proposed that any search for species-specific molecular markers for these three taxonomic species should be continued by applied biologists only if there is better evidence for associating any one of them with phenotypes important for understanding the transmission of Leishmania species in foci of zoonotic cutaneous leishmaniasis.
The laminated layer of hydatid cysts of Echinococcus granulosus represents a considerable amount of parasite material. Its antigenic role, however, is unclear. Extracts of laminated layer taken from sheep cysts were analysed in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS/PAGE) and were found to contain bands at 55 and 25-29 kDa, which reacted with an anti-sheep IgG antibody probe, indicating that these were likely to be host-contaminating components within the layer. However, the same bands were also recognised by a significant proportion of human hydatid patients, particularly by IgG4 antibodies, and not by negative control individuals. These individuals did not recognise immunoglobulin heavy and light chains in a sheep serum extract in the same manner. It seems likely that there are either host or parasite antigenic components at similar molecular weights or that certain parasite antigens may share epitopes with sheep immunoglobulins. The antigens at 25-29 kDa were found to be glycoproteins by lectin blot analysis and may be important markers of disease status.
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