Antibody responses in human cystic hydatid disease to the laminated layer of Echinococcus granulosus

Abstract

The laminated layer of hydatid cysts of Echinococcus granulosus represents a considerable amount of parasite material. Its antigenic role, however, is unclear. Extracts of laminated layer taken from sheep cysts were analysed in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS/PAGE) and were found to contain bands at 55 and 25-29 kDa, which reacted with an anti-sheep IgG antibody probe, indicating that these were likely to be host-contaminating components within the layer. However, the same bands were also recognised by a significant proportion of human hydatid patients, particularly by IgG4 antibodies, and not by negative control individuals. These individuals did not recognise immunoglobulin heavy and light chains in a sheep serum extract in the same manner. It seems likely that there are either host or parasite antigenic components at similar molecular weights or that certain parasite antigens may share epitopes with sheep immunoglobulins. The antigens at 25-29 kDa were found to be glycoproteins by lectin blot analysis and may be important markers of disease status.

Bovine (Bos taurus) humoral immune response against Echinococcus granulosus and hydatid cyst infertility

“…IgGs are present in hydatid fluid and some reports suggest the presence of IgGs at the internal face of the cysts (including both germinal and laminar layers) [Coltorti and Varela‐Diaz, 1972, 1975; Varela‐Diaz and Coltorti, 1972, 1973; Shapiro et al, 1992], although this fact was not studied further. Recently, Taherkhani et al [2007] described that in the laminar layer of ovine hydatid cysts proteins with molecular masses of approximately 55 and 25–29 kDa are present that are recognized by antibodies against ovine IgGs. The same proteins were also recognized by a significant proportion of sera from human hydatid patients, particularly by IgG4 antibodies and not by sera from negative control individuals.…”

Differential expressions of MHC-DQB1 mRNA in Chinese merino sheep infected with Echinococosus granuclosus

“…The disease is characterized by the presence of slow-growing, unilocular cysts in various organs of the intermediate host (Taherkhani et al 2007). The immune response produced by hosts, challenged with E. granuclosus eggs or oncosphere, was different at different stages of infection (Li et al 2011).…”

Immunological diagnosis of human hydatid cyst using Western immunoblotting technique

“…In this regard, it has been shown that bands with molecular weight of about 55, 25, and 29 kDa of laminated layer reacted with anti-sheep IgG. [32]…”