Biochemistry. In the article "Clonal selection and in vivo quantitation of protein interactions with protein-fragment complementation assays" by Ingrid Remy and Stephen W. Michnick, which appeared in number 10, May 11, 1999, of Proc. Natl. Acad. Sci. USA (96, 5394-5399) Immunology. In the article entitled "Murine natural killer cells contribute to the granulomatous reaction caused by mycobacterial cell walls" by I. Apostolou, Y. Takahama, C. Belmant, T. Kawano, M. Huerre, G. Marchal, J. Cui, M. Taniguchi, H. Nakauchi, J.-J. Fournié, P. Kourilsky, and G. Gachelin, which appeared in number 9, April 27, 1999 of Proc. Natl. Acad. Sci. USA (96,(5141)(5142)(5143)(5144)(5145)(5146), the authors request that the following correction be noted: the title should be "Murine natural killer T (NKT) cells contribute to the granulomatous reaction caused by mycobacterial cell walls." Neurobiology. In the articles "An empirical basis for Mach bands" by R. Beau Lotto, S. Mark Williams, and Dale Purves, which appeared in number 9, April 27, 1999, of Proc. Natl. Acad. Sci. USA (96,(5239)(5240)(5241)(5242)(5243)(5244), and "Mach bands as empirically derived associations" by R. Beau Lotto, S. Mark Williams, and Dale Purves, which appeared in number 9, April 27, 1999, of Proc. Natl. Acad. Sci. USA (96,(5245)(5246)(5247)(5248)(5249)(5250), the following correction should be noted. The reproduction of some of the figures in these papers was unsatisfactory due to the presence of moiré patterns and other deficiencies in the published versions. Given the difficulty in faithfully reproducing gradients in print, readers may wish to view the electronic versions of the figures at purveslab.neuro.duke.edu.Psychology. In the article "Spatial attention affects brain activity in human primary visual cortex" by Sunil P. Gandhi, David J. Heeger, and Geoffrey M. Boynton, which appeared in number 6, March 16, 1999, of Proc. Natl. Acad. Sci. USA (96, 3314-3319), due to an error in the PNAS office, a sentence was omitted. The sentence is shown in bold type in context in the complete paragraph below.On the other hand, it is certainly possible that the V1 modulation we observed might have nothing to do with the improved behavioral performance. For example, the memory load differs between the tasks in the main experiment and the spatial uncertainty experiment. In the spatial uncertainty experiment, subjects must remember two speeds instead of one during the 250 msec inter-stimulus interval. This difference in memory load might be causing the improved behavioral performance. Or the improved performance may result from subjects simply ignoring information from the uncued side, and thus may not be causally related to V1 modulation. It is difficult, however, to imagine that such a significant modulation of activity in visual cortex would fail to have consequences on perceptual thresholds. 7610
S U M M A R Y Androgen has been shown to promote the proliferation of prostate cancer through the action of the androgen receptor (AR). Mutation (T877A) of the AR gene found in an androgen-sensitive prostate cancer cell line, LNCaP, has been postulated to be involved in hypersensitivity and loss of specificity for androgen. In the present study, trafficking of AR and AR (T877A) in living prostate and non-prostate cancer cell lines under high and low concentrations of androgen and antiandrogen was investigated by tagging green fluorescent protein (GFP) to the receptors. In the presence of a high concentration of androgen, AR-GFP localized in the nucleus by forming discrete clusters in all cell lines. AR (T877A)-GFP was also translocated to the nucleus in LNCaP and COS-1 cells by the addition of a high concentration of androgen. In contrast, in the presence of a low concentration of androgen, the translocation of AR-GFP and AR (T877A)-GFP was observed in LNCaP cells, but not in COS-1 cells. Upon the addition of antiandrogen, AR-GFP was translocated to the nucleus but did not form subnuclear foci in both COS-1 and LNCaP cells, whereas AR (T877A)-GFP in both cells was translocated to the nucleus with subnuclear foci. The present study demonstrates the differential response of nuclear trafficking of AR and its mutant in prostate cancer cell lines and COS cells, and the subcellular and subnuclear compartmentalization provide important information on the sensitivity of the AR mutation. (J Histochem Cytochem 55:535-544, 2007)
The long term marrow-repopulating ability of mouse bone marrow c-Kit+ Sca-1+ Lin(low/-) cells was studied. Injection of as few as 100 c-Kit+ Sca-1+ Lin(low/-) cells could rescue a lethally irradiated recipient mouse and reconstitute both myeloid and lymphoid cells in their peripheral blood for at least 10 mo. When limiting dilution analysis was performed in the presence of host-derived rescue cells, as low as five c-Kit+ Sca-1+ Lin(low/-) cells were shown to be capable of repopulating lymphohemopoietic cells. Subsequently, we examined whether c-Kit+ Sca-1+ Lin(low/-) cells had a capacity for self-renewal in vivo. After transplantation of 500 c-Kit+ Sca-1+ Lin(low/-) Ly-5.1+ cells into lethally irradiated Ly-5 congenic mice, the expansion of cells with the same phenotypes as the injected cells was monitored. By day 28 post-transplantation, more than 50,000 donor type c-Kit+ Sca-1+ Lin(low/-) Ly-5.1+ cells were found in the spleen and over 18,000 cells were found in bone marrow. The expansion in spleen, however, was transient in that by day 60 cells of the donor phenotype were found only in bone marrow. The c-Kit+ Sca-1+ Lin(low/-) LY-5.1+ cells expanded in spleen or bone marrow contained as many high proliferative potential colony-forming cells as the originally injected cells. They also contained cells with LTRA, but the frequency appeared to be less compared with naive c-Kit+ Sca-1+ Lin(low/-) cells. These data provide evidence that c-Kit+ Sca-1+ Lin(low/-) cells in bone marrow are capable of multilineage differentiation as well as self-renewal and that spleen is a primary site of stem cell expansion after transplantation.
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