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The field of molecular ecology is transitioning from the use of small panels of classical genetic markers such as microsatellites to much larger panels of single nucleotide polymorphisms (SNPs) generated by approaches like RAD sequencing. However, few empirical studies have directly compared the ability of these methods to resolve population structure. This could have implications for understanding phenotypic plasticity, as many previous studies of natural populations may have lacked the power to detect genetic differences, especially over micro-geographic scales. We therefore compared the ability of microsatellites and RAD sequencing to resolve fine-scale population structure in a commercially important benthic invertebrate by genotyping great scallops (Pecten maximus) from nine populations around Northern Ireland at 13 microsatellites and 10 539 SNPs. The shells were then subjected to morphometric and colour analysis in order to compare patterns of phenotypic and genetic variation. We found that RAD sequencing was superior at resolving population structure, yielding higher Fst values and support for two distinct genetic clusters, whereas only one cluster could be detected in a Bayesian analysis of the microsatellite dataset. Furthermore, appreciable phenotypic variation was observed in size-independent shell shape and coloration, including among localities that could not be distinguished from one another genetically, providing support for the notion that these traits are phenotypically plastic. Taken together, our results suggest that RAD sequencing is a powerful approach for studying population structure and phenotypic plasticity in natural populations.

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In the past twenty years marine biotoxin analysis in routine regulatory monitoring has advanced significantly in Europe (EU) and other regions from the use of the mouse bioassay (MBA) towards the high-end analytical techniques such as high-performance liquid chromatography (HPLC) with tandem mass spectrometry (MS). Previously, acceptance of these advanced methods, in progressing away from the MBA, was hindered by a lack of commercial certified analytical standards for method development and validation. This has now been addressed whereby the availability of a wide range of analytical standards from several companies in the EU, North America and Asia has enhanced the development and validation of methods to the required regulatory standards. However, the cost of the high-end analytical equipment, lengthy procedures and the need for qualified personnel to perform analysis can still be a challenge for routine monitoring laboratories. In developing regions, aquaculture production is increasing and alternative inexpensive Sensitive, Measurable, Accurate and Real-Time (SMART) rapid point-of-site testing (POST) methods suitable for novice end users that can be validated and internationally accepted remain an objective for both regulators and the industry. The range of commercial testing kits on the market for marine toxin analysis remains limited and even more so those meeting the requirements for use in regulatory control. Individual assays include enzyme-linked immunosorbent assays (ELISA) and lateral flow membrane-based immunoassays (LFIA) for EU-regulated toxins, such as okadaic acid (OA) and dinophysistoxins (DTXs), saxitoxin (STX) and its analogues and domoic acid (DA) in the form of three separate tests offering varying costs and benefits for the industry. It can be observed from the literature that not only are developments and improvements ongoing for these assays, but there are also novel assays being developed using upcoming state-of-the-art biosensor technology. This review focuses on both currently available methods and recent advances in innovative methods for marine biotoxin testing and the end-user practicalities that need to be observed. Furthermore, it highlights trends that are influencing assay developments such as multiplexing capabilities and rapid POST, indicating potential detection methods that will shape the future market.

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A literature review was conducted to determine the barriers and facilitators to shellfish cultivation using Northern Ireland (NI) as a case study. This review included 23 papers, published in 14 journals, indexed in the Web of Science database. The eligibility criteria included (i) peer‐reviewed articles or websites from key government or agency stakeholders in the aquaculture supply chain; (ii) had a primary focus on shellfish production; and (iii) made reference to the United Kingdom, Ireland or NI. Six key categories were identified as impacting the aquaculture sector: Economic, Environmental, Technical, Welfare, Political and Consumer factors. The most commonly cited codes within these categories included regulations, management decisions, environmental welfare, weather conditions, consumer needs and water quality. Within the scientific literature, the study designs were variable and NI‐specific data were limited. However, the recommendations were relatively consistent. More evidence‐based data collection is needed to better establish the status of the aquaculture industry in this region and to provide more effective management practices and recommendations. The information gained from this review allowed the identification and discussion of the challenges and opportunities for shellfish production worldwide; an insight into production in NI; and preliminary recommendations for improving this industry. As a result, the review offers important foundations and lessons for other aquaculture production units across the world to assess their industry.

BioOne Complete (complete.BioOne.org) is a full-text database of 200 subscribed and open-access titles in the biological, ecological, and environmental sciences published by nonprofit societies, associations, museums, institutions, and presses.

The estuary of the Oued Massa, southern Morocco, has experienced alternating phases of tidal and lagoon conditions. Using field surveys, aerial photographs and rainfall-runoff records the dominant processes and recent evolution of the estuary are described. These data suggest that the character of the estuary has changed since the upstream impoundment of the river by the Youssef Ben Tachfine reservoir. The frequency of low-flows has been significantly increased by river regulation, as has the mean interval between flood events. Using reconstructed flow series it is speculated that lagoon conditions have become more common in the post-impoundment era than at any time since the 1920s. Although increases in winter precipitation projected by the global climate model HadCM2 for 2030-2049 imply a higher frequency of flood flows entering the reservoir in the future, the magnitude and frequency of such inflows are still within historic variability. Nonetheless, higher inflows may produce more frequent spills with concomitant bar breaching and termination of lagoon phases. Despite this, it is argued that the presence of the dam is ultimately of greater consequence to the functioning of the estuary-lagoon system than future precipitation changes over the region.