Sonicated extract of crambe seed meal prepared in the presence of ferrous ion and dithiothreitol enzymatically converts epi-progoitrin to glucose, HSO4−, and a mixture of 1-cyano-2-hydroxy-3,4-epithiobutanes (50–70%) and 1-cyano-2-hydroxy-3-butene (30–50%). A fraction of the extract precipitating between 60 and 70% saturated ammonium sulfate contains thioglucosidase that converts epi-progoitrin essentially to 1-cyano-2-hydroxy-3-butene. Chromatography (on cross-linked dextran) of a 40–60% ammonium sulfate fraction leads to separation of a proteinaceous material (s20 = 2.6 S) that does not hydrolyze epi-progoitrin but, in the presence of thioglucosidase, promotes the formation of 1-cyano-2-hydroxy-3,4-epithiobutanes in amounts proportional to those from crude seed meal extract.
varieties are in good agreement with those for the Swedish varieties, except in the case of lysine, where our values are slightly lower than those reported by Eklund and Agren (1975).Generally plant proteins are deficient in methionine, having less than 2 g/100 g of protein (Van Etten et al., 1967). However, poppy seeds have a higher content which is comparable with the FAO Reference Pattern for this essential amino acid. Thus poppy seed should be good adjunct to vegetable proteins to enhance their nutritive value.
ACKNOWLEDGMENTWe thank Mr. Sitar am, Centre for Cellular and Molecular Biology, Hyderabad, for help in amino acid analysis.
LITERATURE CITEDAOAC "Official Methods of Analysis", 11th ed.
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