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This study was carried out in Plant Tissue Culture Laboratory of the Faculty of Environ. Agric. Sci. El-Arish, North Sinai, Suez Canal University during the period from 2012 to 2015. The present studies were undertaken to look for additional cheap and easily available substitutes of agar to be used in tissue culture media. The obtained results show that MS medium supplemented with BA at 8 mgl-1 resulted in significant increase for all Philodendron growth parameters i.e, number of adventitious shoots/explant, adventitious shoot length (cm), main shoot length (cm) and number of leaves/shoot respectively (4.94, 8.09, 7.60 and 5.33, respectively) after six weeks from culture date comparing with control (MS medium free). However, 12 weeks period was not largely effective in enhancing growth parameters so that, for economical feasibility plants should not be cultured further than 6 weeks. While, in multiplication stage data present that MS medium supplemented with 8mgl-1 with 0.4 NAA mgl-1 produced the highest growth values. Moreover, the combination between agar at 4gl-1 and locust bean (LB) at 4gl-1 as gelling agent produced the maximum growth values. For rooting stage, data clear that number of roots increased and recorded the maximum number (31.67) when shoots of Philodendron were cultured on half MS medium supplemented with 1.0 mgl-1 IBA on the absence of activated charcoal. Finally, Rooted plantlets were acclimatized successively and the highest survivability percentage (100%) was achieved by using the media mixture of vermiculite: peat (1:1) and plants irrigated with quarter MS strength after six weeks.

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