Annona muricata is one of the most important traditional medicinal plants which contains numerous chemicals that exhibit various pharmacological properties. In this study, silver nanoparticles were prepared using A. muricata peel extract as a reducing agent and the effect was enhanced through A. muricata like pharmaceutical activity. AgNPs formation was confirmed by color changes, UV-visible spectroscopy, SEM, DLS, and XRD. The anti-proliferative activity of AgNPs against THP-1, AMJ-13, and HBL cell lines was studied. Apoptotic markers were tested using AO/EtBr staining assay, cell cycle phases using flowcytometry, and the expression of P53. Autophagy takes an essential part in controlling inflammasome activation by primary bone marrow-derived macrophages (BMDMs). We report novel functions for AgNPs-affected autophagy, represented by the control of the release of IL-1β, caspase-1, adaptor protein apoptosis-associated speck-like protein containing a CARD (ASC), and NLRP3 in BMDMs following treatment with LPS+ATP. The current study revealed that the AgNPs inhibited THP-1 and AMJ-13 cell proliferation. Meanwhile, the AgNPs significantly increased autophagy and reduced IL-1b and NLRP3 levels in both in vivo and in vitro models. The secretion of IL-1β was reduced whereas the degradation of NLRP3 inflammasome was enhanced. These findings propose that AgNPs apply an anti-proliferative activity against THP-1 and AMJ-13 cells through the stimulation of apoptosis via mitochondrial damage and induction of p53 protein pathway. In addition, AgNP-induced autophagy reduced the levels of IL-1β and NLRP3 inflammasome activation. This indicated that the AgNPs augment autophagy controlled by the IL-1β pathway via two different novel mechanisms. The first one is regulating activation of the IL-1 β, caspae-1, and ASC, while the second is NLRP3 targeting for lysosomal degradation. Overall, this study suggests that AgNPs could be a potent therapy for various types of cancer and an alternative treatment for preventing inflammation via enhancing autophagy.
The effects of climate temperature and water stress on growth and several stress markers were investigated in sweet basil plants. Some growth parameters (shoot length and number of leaves) and photosynthetic chlorophyll contents were determined every two days during plant growth, and foliage leaf material was collected after 15 and 21 days of treatment. Both climate temperature and water stress inhibited sweet basil plant growth; especially, total chlorophyll levels were decreased significantly in response to high-temperature treatments. Under strong stresses, basil plants induced the synthesis and accumulation of glycine betaine (GB) as a secondary osmolyte, although at less content when compared with the proline content under the same stress conditions. Proline concentrations particularly increased in leaves of both basil stressed plants, accomplishing levels high enough to play a crucial role in cellular osmoregulation adjustment. Stress-induced accumulation of these antioxidant compounds was detected in sweet basil. Therefore, it appears that sweet basil-treated plants are able to synthesize antioxidant compounds under strong stress conditions. On the other hand, total sugar concentrations decreased in stress-treated basil plants. Both temperature and water stress treatments caused oxidative stress in the treated plants, as indicated by a significant increment in malondialdehyde (MDA) concentrations. An increase in total phenolic and flavonoid concentrations in response to water stress and a highly significant decrease in carotenoid concentrations in basil leaves were observed; flavonoids also increased under high climate temperature conditions.
This paper describes the preparation, characterization, and evaluation of honey/tripolyphosphate (TPP)/chitosan (HTCs) nanofibers loaded with capsaicin derived from the natural extract of hot pepper (Capsicum annuumL.) and loaded with gold nanoparticles (AuNPs) as biocompatible antimicrobial nanofibrous wound bandages in topical skin treatments. The capsaicin and AuNPs were packed within HTCs in HTCs-capsaicin, HTCs-AuNP, and HTCs-AuNPs/capsaicin nanofibrous mats. In vitro antibacterial testing against Pasteurella multocida, Klebsiella rhinoscleromatis,Staphylococcus pyogenes, and Vibrio vulnificus was conducted in comparison with difloxacin and chloramphenicol antibiotics. Cell viability and proliferation of the developed nanofibers were evaluated using an MTT assay. Finally, in vivo study of the wound-closure process was performed on New Zealand white rabbits. The results indicate that HTCs-capsaicin and HTCs-AuNPs are suitable in inhibiting bacterial growth compared with HTCs and HTCs-capsaicin/AuNP nanofibers and antibiotics (P < 0.01). The MTT assay demonstrates that the nanofibrous mats increased cell proliferation compared with the untreated control (P < 0.01). In vivo results show that the developed mats enhanced the wound-closure rate more effectively than the control samples. The novel nanofibrous wound dressings provide a relatively rapid and efficacious wound-healing ability, making the obtained nanofibers promising candidates for the development of improved bandage materials.
Recently, there has been a growing interest in research on nanofibrous scaffolds developed by electrospinning bioactive plant extracts. In this study, the extract material obtained from the medicinal plant Inula graveolens (L.) was loaded on polycaprolactone (PCL) electrospun polymeric nanofibers. The combined mixture was prepared by 5% of I. graveolens at 8% (PCL) concentration and electrospun under optimal conditions. The chemical analysis, morphology, and crystallization of polymeric nanofibers were carried out by (FT-IR) spectrometer, scanning electron microscopy (SEM), and XRD diffraction. Hydrophilicity was determined by a contact angle experiment. The strength was characterized, and the toxicity of scaffolds on the cell line of fibroblasts was finally investigated. The efficiency of nanofibers to enhance the proliferation of fibroblasts was evaluated in vitro using the optimal I. graveolens/PCL solutions. The results show that I. graveolens/PCL polymeric scaffolds exhibited dispersion in homogeneous nanofibers around 72 ± 963 nm in the ratio 70/30 (V:V), with no toxicity for cells, meaning that they can be used for biomedical applications.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.