A series of 4,6-disubstituted 2-(5-nitrofurfurylidenehydrazino)-1,3,5-triazines has been synthesized. Some of these compounds possessed good antibacterial activity against Mycobacterium tuberculosis in vitro and moderate activity in vivo (the activity in vivo was observed only with high dosages compared with compounds in clinical use and the results were also irregular). The most active compounds are triazinylhydrazones of 5-nitro-2-furaldehyde in which the triazine ring is substituted with two amino-groups, at least one of which is an (a-branched chain alky1)-or cycloalkylamine. Structure-antituberculosis activity relations for these compounds are discussed.
Only minimal differences between endogenous and glucose respiration were demonstrable with 0.03 M phosphate buffer at pH 7. The addition of 1 ml of trace metals (Table 1) or a modified Ringer's solution (Table 2) enhanced glucose respiration markedly. The a priori hypothesis that magnesium ions were largely responsible for this stimulation was tested by adding 1 ml of the trace-metal mixture and 38.2 mg MgSO4 .7H20 to 100 ml 0.03 M phosphate buffer at pH 7. This variation proved to be superior to the previous modifications, and RQ's within 5% of theoretical were obtained with several carbon sources. With this buffer, results represented in Fig. 1 and 2 were achieved. They indicate that several carbohydrates and inter-mediates of carbohydrate metabolism were oxidized by C. longisporum without immediate prior experience. Inspection of these curves also suggests the ability of the fungus to adapt to certain of these other substrates. It is noteworthy that earlier in this investigation phosphate concentration appeared not to influence the glucose respiration of C. Iongisporum. Exhaustive shaking of the mycelium in distilled water was required before the need for phosphate became manifest, suggesting intracellular metaphosphate stores. This study would suggest that other filamentous fungi may be studied similarly after their specific metal requirements for manometric work have been established.
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