An outbreak of caprine tuberculosis caused by Mycobacterium bovis was disclosed in June 2008, affecting goats of the golden Guernsey breed kept on 10 separate smallholdings in south-west Wales and the west of England. Following the initial diagnosis at postmortem examination, 30 goats that reacted positively to the single intradermal comparative cervical tuberculin (SICCT) test, together with five in-contact animals, were euthanased and subjected to postmortem examination and mycobacterial culture. Spoligotyping and variable number tandem repeat analysis of isolates showed that they were all of the same genotype, endemic to south-west Wales. Retrospective movement tracings identified a goat herd in south-west Wales, by then completely dispersed, as the probable common source of infection. There was a perfect correlation between the SICCT test and culture results in all slaughtered goats. Grossly visible tubercular lesions were observed at postmortem examination in all but one reactor.

SUMMARY Collagens type I, II, III, IV, and V and the minor cartilage collagens, la 2a 3a, C-PS 1, and C-PS 2, were purified, antibodies raised, and then used in immunofluorescence studies on bovine nasal cartilage (BNC). Punctate This report describes the localisation of these collagens in BNC by immunofluorescence.Materials and methods Collagens. These were isolated from various bovine tissues after limited pepsin digestion. Type II, la 2a 3a, C-PS 1, and C-PS 2 collagens were isolated from adult nasal cartilage as described by Ayad et al.7 8 Types I and III collagen were prepared from placental membranes according to Epstein.' Types IV and V collagen were isolated from placental villi and membrane respectively by the method of Abedin et 575 on 11 May 2018 by guest. Protected by copyright.

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SUMMARY Native la 2ca 3a collagen (50 ,ug