Soil salinity affects large areas of cultivated land, causing significant reductions in crop yield globally. The Na + toxicity of many crop plants is correlated with overaccumulation of Na + in the shoot. We have previously suggested that the engineering of Na + exclusion from the shoot could be achieved through an alteration of plasma membrane Na + transport processes in the root, if these alterations were cell type specific. Here, it is shown that expression of the Na + transporter HKT1;1 in the mature root stele of Arabidopsis thaliana decreases Na + accumulation in the shoot by 37 to 64%. The expression of HKT1;1 specifically in the mature root stele is achieved using an enhancer trap expression system for specific and strong overexpression. The effect in the shoot is caused by the increased influx, mediated by HKT1;1, of Na + into stelar root cells, which is demonstrated in planta and leads to a reduction of root-to-shoot transfer of Na + . Plants with reduced shoot Na + also have increased salinity tolerance. By contrast, plants constitutively expressing HKT1;1 driven by the cauliflower mosaic virus 35S promoter accumulated high shoot Na + and grew poorly. Our results demonstrate that the modification of a specific Na + transport process in specific cell types can reduce shoot Na + accumulation, an important component of salinity tolerance of many higher plants. INTRODUCTIONSoil salinity affects large areas of cultivated land in more than 100 countries (Rengasamy, 2006). Increased soil salinity negatively affects the growth of many crop plants, and the continued salinization of arable land provides an increasing threat to global crop production, especially in irrigated systems (Munns and Tester, 2008). Increasing the salinity tolerance of crop plants will provide an important contribution to the maintenance of crop yields.The Na + toxicity of many crop plants is correlated with overaccumulation of Na + in the shoot (Munns, 1993(Munns, , 2002Tester and Davenport, 2003;Møller and Tester, 2007). Na + is taken up from the soil by the plant root system and transported to the shoot in the transpiration stream . Shoot Na + accumulation is the net result of distinct Na + transport processes occurring in different organs and cell types , and each of these processes contributes to the salinity tolerance of a plant.Such Na + transport processes include passive influx of Na + into the root system, which is likely to be mediated by nonselective cation channels (Davenport and Tester, 2000;, with cyclic nucleotidegated channels and Glu receptors being likely candidate gene families encoding these proteins Roy et al., 2008). The HKT family of ion transporters originally was named for the high-affinity potassium transporter properties of the first member of this family that was isolated, but it is more complex than originally realized, as the affinity and selectivity of many proteins encoded by members of this gene family are different to that indicated by the name. As has been shown for HKT2;1 and HKT2;2 in rice (Oryza sa...
We report physiological and anatomical characteristics of water transport across roots grown in soil of two cultivars of grapevine (Vitis vinifera) differing in response to water stress (Grenache, isohydric; Chardonnay, anisohydric). Both cultivars have similar root hydraulic conductances (L o ; normalized to root dry weight) that change diurnally. There is a positive correlation between L o and transpiration. Under water stress, both cultivars have reduced minimum daily L o (predawn) attributed to the development of apoplastic barriers. Water-stressed and well-watered Chardonnay had the same diurnal change in amplitude of L o , while water-stressed Grenache showed a reduction in daily amplitude compared with well-watered plants. Hydraulic conductivity of root cortex cells (L pcell ) doubles in Chardonnay but remains unchanged in Grenache. Of the two most highly expressed plasma membrane intrinsic protein (PIP) aquaporins in roots (VvPIP1;1 and VvPIP2;2), only VvPIP2;2 functions as a water channel in Xenopus laevis oocytes. VvPIP1;1 interacts with VvPIP2;2 to induce 3-fold higher water permeability. These two aquaporins are colocated in the root from in situ hybridization and immunolocalization of VvPIP1 and VvPIP2 subfamily members. They occur in root tip, exodermis, root cortex (detected up to 30 mm), and stele. VvPIP2;2 mRNA does not change diurnally or with water stress, in contrast to VvPIP1;1, in which expression reflects the differences in L o and L pcell between cultivars in their responses to water stress and rewatering. VvPIP1;1 may regulate water transport across roots such that transpirational demand is matched by root water transport capacity. This occurs on a diurnal basis and in response to water stress that corresponds to the difference in drought tolerance between the cultivars. Root hydraulic conductance is usually lowest within the liquid component of the soil-plant-air continuum. The hydraulic conductance of roots can be highly variable in both time and space, which will affect soilwater extraction and shoot water status (Steudle and Peterson, 1998;Steudle, 2000aSteudle, , 2000b. Steudle (2000aSteudle ( , 2000b explains variation in root hydraulic conductivity (L p ; hydraulic conductance normalized to root surface area) in terms of the composite transport model based on the composite anatomical structure of roots, where water can move radially toward the xylem along three pathways: the apoplastic, symplastic, and transcellular. The symplastic and transcellular pathways are difficult to separate experimentally and are collectively considered as the cell-to-cell pathway (Steudle, 2000b). The extent to which water flow predominates in either pathway varies according to the relative hydraulic conductances of the pathways and the relative magnitude of hydrostatic versus osmotic gradients (Steudle, 2000a; Bramley et al., 2007b). Apoplastic flow can be altered irreversibly by anatomical changes, including Casparian bands and suberin lamellae (Steudle and Peterson, 1998). The conductance of the cell...
Both limiting and toxic soil concentrations of the essential micronutrient boron represent major limitations to crop production worldwide. We identified Bot1, a BOR1 ortholog, as the gene responsible for the superior boron-toxicity tolerance of the Algerian barley landrace Sahara 3771 (Sahara). Bot1 was located at the tolerance locus by high-resolution mapping. Compared to intolerant genotypes, Sahara contains about four times as many Bot1 gene copies, produces substantially more Bot1 transcript, and encodes a Bot1 protein with a higher capacity to provide tolerance in yeast. Bot1 transcript levels identified in barley tissues are consistent with a role in limiting the net entry of boron into the root and in the disposal of boron from leaves via hydathode guttation.
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