Boron-Toxicity Tolerance in Barley Arising from Efflux Transporter Amplification

Sutton, Tim; Baumann, Ute; Hayes, J. F.; Collins, Nicholas C.; Shi, Bu Jun; Schnurbusch, Thorsten; Hay, Alison; Mayo, Gwenda M.; Pallotta, Margaret; Tester, Mark; Langridge, Peter

doi:10.1126/science.1146853

Cited by 414 publications

(361 citation statements)

References 17 publications

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“…The first B toxicity tolerance gene identified in plants was the barley 4H QTL gene Boron tolerance 1, Bot1 (Reid 2007, named as such by Sutton et al 2007 following the accepted rules for nomenclature and gene symbolization in barley (Franckowiak et al 1997). Bot1 was identified using an approach combining positional cloning and reverse genetics.…”

Section: B Tolerance In Barleymentioning

confidence: 99%

Boron toxicity tolerance in wheat and barley: Australian perspectives

2010

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Section: B Tolerance In Barleymentioning

confidence: 99%

Boron toxicity tolerance in wheat and barley: Australian perspectives

2010

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“…In particular, the entire chromosome-level collinearity was observed between barley chromosome 3H and rice chromosome 1 (Figure 4). Comparative genomic information can aid systematic marker generation even when the corresponding interval in the rice genome lacks a barley ortholog (Komatsuda et al, 2007;Sutton et al, 2007). This approach is particularly useful for the identification of functional homologs in both species (Inukai et al, 2006) and identification of candidate orthologs in barley.…”

Section: K Sato Et Almentioning

confidence: 99%

A high-density transcript linkage map of barley derived from a single population

2009

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A high-resolution transcript linkage map of barley was created using a single doubled haploid (DH) mapping population, only 3 0 -end expressed sequence tags (ESTs) and only PCR-based assays. Cultivar 'Haruna Nijo' and an ancestral wild-form accession 'H602' were used as EST donors and crossing parents of the mapping population. Of the 10 366 primer sets developed from a non-redundant set of 3 0 EST sequences, 7700 sets generated useful amplicons and 3975 (52%) showed polymorphisms between the mapping parents. Of these, 2890 (28% of the total) were mapped by single nucleotide polymorphisms (1717), cleaved amplified polymorphic sequence (933) and INDELs (240). The present work involves an estimated 9% of the genes of barley. Of the mapped ESTs, 2689 (93%) are formatted in the Affymetrix Barley 1 GeneChip and full-length cDNA sequences are available for 1039 (36%). Mapped ESTs show highest similarity with sequences in the wheat gene index (93%) and moderate similarity with rice (50%). Comparison of mapped EST positions and the rice pseudomolecule indicated collinear regions between two species; these are particularly conserved for the entire barley chromosome 3H and rice chromosome 1. These mapped genes, together with a systematically developed set of genetic resources, will make it easier to directly clone genes showing simple inheritance and to determine the genetic basis of complex traits. The information will contribute to the development of a framework for the physical mapping of barley, which is necessary for genome sequencing.

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“…The size of a sliding window was chosen to include 30 adjacent positions in the genetic map. Yan et al, 2006;Komatsuda et al, 2007;Sutton et al, 2007;Taketa et al, 2008;Nair et al, 2010;Ramsay et al, 2011) were searched for the presence of flanking or cosegregating markers and gene sequences with BLASTN (Altschul et al, 1990), or BAC clone identifiers from the original publications were identified in the FPC database of fingerprinted contigs.…”

Section: Distribution Of Bac Librariesmentioning

confidence: 99%

A Sequence-Ready Physical Map of Barley Anchored Genetically by Two Million Single-Nucleotide Polymorphisms

et al. 2013

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Barley (Hordeum vulgare) is an important cereal crop and a model species for Triticeae genomics. To lay the foundation for hierarchical map-based sequencing, a genome-wide physical map of its large and complex 5.1 billion-bp genome was constructed by high-information content fingerprinting of almost 600,000 bacterial artificial chromosomes representing 14-fold haploid genome coverage. The resultant physical map comprises 9,265 contigs with a cumulative size of 4.9 Gb representing 96% of the physical length of the barley genome. The reliability of the map was verified through extensive genetic marker information and the analysis of topological networks of clone overlaps. A minimum tiling path of 66,772 minimally overlapping clones was defined that will serve as a template for hierarchical clone-by-clone map-based shotgun sequencing. We integrated whole-genome shotgun sequence data from the individuals of two mapping populations with published bacterial artificial chromosome survey sequence information to genetically anchor the physical map. This novel approach in combination with the comprehensive whole-genome shotgun sequence data sets allowed us to independently validate and improve a previously reported physical and genetic framework. The resources developed in this study will underpin fine-mapping and cloning of agronomically important genes and the assembly of a draft genome sequence.

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Boron-Toxicity Tolerance in Barley Arising from Efflux Transporter Amplification

Cited by 414 publications

References 17 publications

Boron toxicity tolerance in wheat and barley: Australian perspectives

Boron toxicity tolerance in wheat and barley: Australian perspectives

A high-density transcript linkage map of barley derived from a single population

A Sequence-Ready Physical Map of Barley Anchored Genetically by Two Million Single-Nucleotide Polymorphisms

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