ABSTRAKTanaman mentimun dengan gejala daun menguning mulai banyak ditemukan pada sejumlah pertanaman mentimun di Desa Apuan dan Desa Bangli, Provinsi Bali. Penyakit ini diduga berasosiasi dengan Begomovirus karena gejalanya mirip dengan infeksi Begomovirus yang dilaporkan terjadi pada tanaman mentimun di Jawa. Selain itu, kutukebul (Bemisa tabaci) ditemukan di lapangan. Penelitian ini bertujuan mengidentifikasi penyebab penyakit daun kuning pada tanaman mentimun. Deteksi dan identifikasi virus dilakukan dengan teknik polymerase chain reaction menggunakan pasangan primer universal Begomovirus, SPG1/SPG2. Pita DNA berukuran 912 pb berhasil diamplifikasi dari sampel tanaman. Analisis hasil sikuensing nukleotida menunjukkan bahwa Begomovirus yang menginfeksi tanaman mentimun di Bali mempunyai nilai kemiripan tertinggi (91%) dengan Squash leaf curl China virus (SLCCNV) isolat Malaysia. Penelitian ini merupakan laporan pertama infeksi SLCCNV di Bali. ABSTRACTLeaf yellowing symptoms was commonly found in cucumber plants in Bali provinces, i.e. in Apuan and Bangli villages recently. Begomovirus infection is suspected as the causal agent, due to similar symptoms previously reported from cucumber plants in Java. In addition, Bemisia tabaci was observed in the field. The objective of this research was to identify the causal agent of leaf yellowing disease of cucumber in Bali. Virus detection and identification was conducted by polymerase chain reaction method using universal primers for Begomovirus, i.e. SPG1/SPG2. DNA fragment of 912 bp in size was successfully amplified from leaf samples. Analysis of nucleotide sequencing indicated that Begomovirus infecting cucumber plants in Bali has the highest homology (91%) with Squash leaf curl China virus (SLCCNV) isolate from Malaysia. This is the first report of SLCCNV infection in Bali.
Banana bunchy top virus (BBTV) mostly infects Musa spp. which causes banana bunchy top disease (BBTD), the most devastating viral disease in banana cultivation all over the world. During field survey in Sekar Bumi Tropical Farm located in Kerta Village, 15 heliconia plant samples showed green streak on lamina leaves that similar to common BBTD symptoms were collected. Further research was then conducted to identify molecular characteristic of BBTD in heliconia showing green streak. Molecular identification was conducted using polymerase chain reaction (PCR). Specific primers to amplified DNA target were used in this study: mRep/F (5’-GCGTGAAACGCACAAAAGGCC-3’) and mRep/R (5’-GCATACGTTGTCAAACCTTCTCCTC-3’). The expected 240 bp fragment target of partial master replication (mRep) gene was successfully amplified from ten out of 15 samples. Sequence analysis confirmed that the symptomatic heliconia samples were infected with BBTV and falls into the same clade with BBTV from the Asian Group.
Bunchy top symptoms on banana has been reported in Bali Island since early 2011. Symptoms variation were observed in the field similar to infection of Banana bunchy top virus (BBTV). The identity of the BBTV in Bali on the basis of DNA-S nucleotide sequence has not been studied, therefore research was conducted to identify the species of BBTV infecting local banana in Bali based on sequence analysis. Research activities were initiated by collecting field samples from several local banana growing areas in Bali Island. Incidence of bunchy top disease in all locations reached 8% to 44% with disease severity ranged from 2.6% to 30%. Identification of BBTV from field samples were done by polymerase chain reaction using specific primers for BBTV (CPF/CPR) followed by sequence analysis of amplified DNA target. Specific BBTV DNA fragment was successfully amplified from 10 field samples; sequence analysis of DNA fragments showed their highest homology with BBTV. In addition the phylogenetic analysis confirmed the close relationship of BBTV isolates from Bali with various BBTV isolates from Indonesia and other isolates from the Asian group in GeneBank.
Tujuan: Dengan mengadopsi sistem pertanian yang selaras alam melalui pendekatan sistem pertanian organik diharapkan menghasilkan komoditas yang sehat dan aman untuk dikonsumsi dan memiliki nilai jual yang lebih tinggi sehingga ke dua misi tersebut dalam jangka panjang dapat dicapai. Metode Penelitian: Penelitian mengenai identifikasi potensi dan pengembangan sistem pertanian organik menuju Bali pulau organik telah dilakukan diseluruh wilayah kabupaten dan Kota di Provinsi Bali sejak bulan Mei – Desember 2019. Penelitian dilakukan melalui survei lapang dan hasilnya disepakati melalui FGD bersama para stakeholder. Temuan: Pengembangan sistem pertanian organik potensial dilakukan hampir seluruh kabupaten di Bali kecuali di Kabupaten Klungkung dan Kota Denpasar. Implikasi: Inovasi yang dihasilkan dari penelitian ini adalah sistem database pertanian organik Provinsi Bali yang online, terintegrasi, kompak, ringan, dan mudah dioperasikan.
ABSTRAKPenyakit layu stewart merupakan penyakit serius pada tanaman jagung manis (Zea mays). Gejala khas penyakit ini ialah garis berwarna hijau pucat sampai kekuningan yang sejajar dengan tulang daun. Gejala seperti ini ditemukan pada daun jagung manis di daerah Denpasar, Tabanan, Gianyar dan Karangasem pada tahun 2015. Deteksi patogen dengan metode polymerase chain reaction dilakukan menggunakan DNA total yang diisolasi dari daun tanaman bergejala dan pasangan primer CPSL1/ CPSR2c. Amplikon dengan ukuran ~1100 pb berhasil diperoleh dari sampel asal Denpasar. Analisis sikuen nukleotida memastikan bahwa gejala penyakit layu stewart tersebut disebabkan oleh bakteri Pantoea stewartii subsp. stewartii. Hasil analisis sikuen menunjukkan bahwa P. stewartii subsp. stewartii isolat Bali memiliki tingkat homologi yang tinggi (98.97-99.08%) dan berada dalam satu clade dengan isolat asal Kanada, Amerika Serikat, dan Jepang. Ini merupakan laporan pertama mengenai P. stewartii subsp. stewartii yang menginfeksi tanaman jagung di Provinsi Bali.Kata kunci: analisis sikuen, nukleotida, Pantoea, Zea mays ABSTRACT Stewart's wilt is a serious disease of sweet corn (Zea mays). The typical symptoms of the disease are pale-green to yellow linear streaks parallel to the veins. The symptoms were observed on sweet corn in Denpasar, Tabanan, Gianyar, and Karangasem areas during a survey in 2015. Pathogen detection based on a polymerase chain reaction was carried out using total DNA obtained from symptomatic leaf samples and the pairs of primers, CPSL1/CPSR2c. The expected sized (~1100 bp) amplicon was detected in samples from Denpasar. Sequence analysis confirmed that Stewart's wilt disease symptoms are caused by Pantoea stewartii subsp. stewartii. Nucleotide sequence and phylogenetic analysis showed that P. stewartii subsp. stewartii from Bali has high homology (98.97-99.08 %) and placed in the same clade with isolates from Canada, USA and Japan. This is the first report of P. stewarti subsp. stewartii on corn in Bali.
Sycanus aurantiacus Ishikawa & Okajima, found in Bali, was first described in 2007 as a new harpactorine species based on morphological and biological characteristics; however, its genome has not yet been sequenced. In this study, we examine the mitochondrial cytochrome oxidase subunit I (MtCOI) nucleotide sequence of S. aurantiacus in order to determine whether it represents a new harpactorine species. A sample from Pancasari, Bali, Indonesia was collected at the same location S. aurantiacus was first discovered in 2007. The selected mtCOI gene (650 bp) was successfully amplified using mtCOI primer pairs LCO1490 and HCO2198, and the resulting MtCOI sequence of the S. aurantiacus sample was compared with those from other hapactorine species recorded in GenBank. This comparison revealed low genetic similarity between S. aurantiacus and most other harpactorine species worldwide, except for the Genus Sycanus (JQ888697) from USA whose mtCOI shares approximately 91% similarity with the Pancasari sample. Phylogenetic analysis indicated a close genetic relationship between Sycanus from Bali and the Genus Sycanus (JQ888697) from the USA. The mtCOI sequence of S. aurantiacus had not been recorded previously, and our comparison with existing Sycanus sequences provides support to the understanding that S. aurantiacus is indeed its own species.
Chili is a type of plant that can grow in various types of area, in lowland and highland areas and also can adapt well at a temperature of 24-27 ˚C. The purpose of this research in order to know the influence and effectiveness of the packages technology of Trichoderma sp. mixed with compost, rain shelter and pesticides to control the development of pests in cayenne chili plants and long chili in the highland area. This research was conducted from September 2017 to February 2018, in Pancasari Village, Buleleng Regency, Bali. The study used a Factorial Randomized Block Design with two factors and four times replications. The first factor was chili type (cayenne chili and long chilli), and the second factor was the use of technologies, compost plus Trichoderma sp., rain shelter, and pesticide. The variable was observed in this research were population of aphis, percentage of fruit fly and yields. The results showed that all treatment technologies effectively suppressed pests development when compared with control. The result show that the damage population of the highest aphis pest was found in control about 194 of Aphids, and population of aphids on pesticide treatment were not found. The result show that damage percentage of the highest fruit fly pest was found in control treatment with the percentage of 76,55% while the lowest percentage was found in pecticide treatment. The rain shelter treatment showed the highest yields were reached 405,87 gr and the control treatment showed the lowest yields were reached 12,03 gr.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.