PENDAHULUAN Pinus (Pinusmerkusii) merupakan tumbuhanGymnospermae atau berbiji terbuka yang memiliki tajuk pohon berbentuk cemaraatau kerucut dan daun berbentuk jarum. Pinus termasuk jenis yang cepat tumbuh dan dapat tumbuh baik pada tanah yang kurang subur serta tidak memerlukan tempat tumbuh dengan persyaratan khusus.Hampir semua bagian pohon dapat dimanfaatkan, antara lain bagian batangnya dapat disadap untuk diambil ABSTRACTThe aims of Genetic study of pinus identified stand in Unhas Experimental Forest is to analyses of genetic characteristics of stand, based on RAPD (Random Amplified Polymorphic DNA) marker. The study was conducted in Biotechnology and Tree Breeding Laboratory, Forestry Faculty, Hasanuddin University. The method are DNA isolation, Primers selection and RAPD analyses. DNA analyses of Pine with ten RAPD marker showed number of alel varietied and polymorphic. Coefficient of similarity in population have number of 0.15-0.73.Highest genetic distance is 0.9630 and lowest genetic distance is 0.2698. Number of genetic diversity of Pine in Experimental Forest Hasanuddin University is 0,489 and categorized highly.
This research aimed to identify the mycorrhiza types that associated in roots and soil on Palaquium sp. plant. This research has implemented on December 2017 until February 2018. This research procedures made by two stages includes collecting soil and root samples on Palaquium sp plant on Ko'mara Community Forest, Takalar District. Therefore, the second step is isolation, identification, and observing colonization of mycorrhiza spores in the Integrated Laboratory and Biotechnology and Tree Breeding Laboratory, Faculty of Forestry, Hasanuddin University, Makassar. The results showed types of spore that found were Glomus, Gigaspora and Acaulospora. Glomus spore has highest relative abundance in root samples whereas on soil samples were Glomus, Gigaspora, and Schleroderma with Glomus spores has highest relative abundance in. The colonization percentage on site was intermediate.
Polymerase Chain Reaction (PCR)-based molecular techniques have been used to detect the polymorphism in plants. The utilization of molecular markers plays essential role in germplasm characterization and plant breeding since the information of DNA marker technology can be exchanged between laboratories and should have standard method to be reproducible. The molecular aspect has been commonly linked to DNA isolation protocol and polymorphic molecular marker, thus can be used for molecular research recommendation purposes. The objectives of this study were to evaluate the capability of microsatellite marker of Ebenaceae Family for amplifying Ebony DNA, and to determine the appropriate PCR annealing temperatures. The DNA isolation of Ebony leaves from Experimental Forest of Hasanuddin University Provenance was carried out using Genomic DNA Mini Kit (Plant) Geneaid protocol. Nine of seventeen selected primers from the Genus Diospyros were able to amplify Ebony DNA. Amplification products produced polymorphic bands with different annealing temperatures (ranged from 53 to 56°C). These nine polymorphic primers will be recommended to use for future studies in genetic diversity as well as pollen dispersal pattern analyses.
Mycorrhizas are the symbiotic associations between plant and fungi. Mycorrhizal fungi always associate with the roots of higher plants, indeed over 90% of plant species, including forest trees, wild grasses and many crops. This study aims to determine the type of spores associated with Gmelina arborea stands. This research consisted of collecting root samples, identifying fungal spores in root samples and observing arbuscular mychorriza fungal infections. The results showed that the types of spores observed in root samples were Glomus, Gigaspora, and Scutellospora. The percentage of colonization on both sites is medium.
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